Succinic dehydrogenase (SDH) was localized in the mitochondria of Sarcoma 37 ascites tumor cells by the use of tetranitro-BT (TNBT) and nitro-BT (NBT) in smear preparations. Results with each tetrazolium salt as electron acceptor were evaluated with respect to: (a) size and shape of the formazan precipitate relative to standard mitochondrial morphology; (b) crystallization phenomena of reduced dye; (c) lipid adsorption of formazan. The association of formazan- or iron hematoxylin-stained mitochondria with lipid droplets within the cells was investigated, as was also the influence of formalin fixation, with and without cold acetone pretreatment, on mitochondrial morphology and enzymatic staining. Data from these studies appear to indicate that TNBT is more suitable than NBT for use as a cytochemical reagent in oxidative and/or dehydrogenase enzyme histochemistry and cytochemistry.
Article|
March 01 1963
THE USE OF TETRANITRO-BLUE TETRAZOLIUM FOR THE CYTOCHEMICAL LOCALIZATION OF SUCCINIC DEHYDROGENASE : Cytochemical and Cytological Studies of Sarcoma 37 Ascites Tumor Cells
Charles G. Rosa,
Charles G. Rosa
From the Daniel Baugh Institute of Anatomy, Jefferson Medical College of Philadelphia, and Central Research Laboratories, Borden Chemical Company, Philadelphia
Search for other works by this author on:
Kwan-Chung Tsou
Kwan-Chung Tsou
From the Daniel Baugh Institute of Anatomy, Jefferson Medical College of Philadelphia, and Central Research Laboratories, Borden Chemical Company, Philadelphia
Search for other works by this author on:
Charles G. Rosa
From the Daniel Baugh Institute of Anatomy, Jefferson Medical College of Philadelphia, and Central Research Laboratories, Borden Chemical Company, Philadelphia
Kwan-Chung Tsou
From the Daniel Baugh Institute of Anatomy, Jefferson Medical College of Philadelphia, and Central Research Laboratories, Borden Chemical Company, Philadelphia
Received:
March 01 1962
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright, 1963, by The Rockefeller Institute Press
1963
J Cell Biol (1963) 16 (3): 445–454.
Article history
Received:
March 01 1962
Citation
Charles G. Rosa, Kwan-Chung Tsou; THE USE OF TETRANITRO-BLUE TETRAZOLIUM FOR THE CYTOCHEMICAL LOCALIZATION OF SUCCINIC DEHYDROGENASE : Cytochemical and Cytological Studies of Sarcoma 37 Ascites Tumor Cells . J Cell Biol 1 March 1963; 16 (3): 445–454. doi: https://doi.org/10.1083/jcb.16.3.445
Download citation file: