Yeast mitochondrial fission is a multistep process during which the dynamin-related GTPase, Dnm1p, assembles into punctate structures that associate with the outer mitochondrial membrane and mediate mitochondrial division. Steps in the Dnm1p-dependent process of fission are regulated by the actions of the WD repeat protein, Mdv1p, and the mitochondrial outer membrane protein, Fis1p. Our previous studies suggested a model where Mdv1p functions to regulate fission at a post-Dnm1p assembly step and Fis1p functions at two distinct steps, at an early point, to regulate Dnm1p assembly, and later, together with Mdv1p, to facilitate Dnm1p-dependent mitochondrial fission. To test this model, we have examined the physical and functional relationship between Mdv1p and Fis1p and present genetic, biochemical, and two-hybrid data indicating that a Fis1p–Mdv1p complex is required to regulate mitochondrial fission. To further define the role of Mdv1p in fission, we examined the structural features of Mdv1p required for its interactions with Dnm1p and Fis1p. Data from two-hybrid analyses and GFP-tagged domains of Mdv1p indicate that it contains two functionally distinct domains that enable it to function as a molecular adaptor to regulate sequential interactions between Dnm1p and Fis1p and catalyze a rate-limiting step in mitochondrial fission.
Skip Nav Destination
Article navigation
5 August 2002
Report|
August 05 2002
The WD repeat protein, Mdv1p, functions as a molecular adaptor by interacting with Dnm1p and Fis1p during mitochondrial fission
Quinton Tieu,
Quinton Tieu
Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616
Search for other works by this author on:
Voytek Okreglak,
Voytek Okreglak
Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616
Search for other works by this author on:
Kari Naylor,
Kari Naylor
Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616
Search for other works by this author on:
Jodi Nunnari
Jodi Nunnari
Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616
Search for other works by this author on:
Quinton Tieu
Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616
Voytek Okreglak
Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616
Kari Naylor
Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616
Jodi Nunnari
Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616
Address correspondence to Jodi Nunnari, Section of Molecular and Cellular Biology, University of California, Davis, 3220 Briggs Hall/LSA, Davis, CA 95616-8535. Tel.: (530) 754-9774. Fax: (530) 752-7522. E-mail: [email protected]
Q. Tieu and V. Okreglak contributed equally to this work.
*
Abbreviations used in this paper: AD, activating domain; BD, binding domain; C-C, coiled coil; DSP, dithiobis(succinimidylpropionate); MBP, maltose-binding protein; NTE, NH2-terminal extension; SB, sorbitol buffer.
Received:
May 08 2002
Revision Received:
June 19 2002
Accepted:
June 21 2002
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2002
J Cell Biol (2002) 158 (3): 445–452.
Article history
Received:
May 08 2002
Revision Received:
June 19 2002
Accepted:
June 21 2002
Citation
Quinton Tieu, Voytek Okreglak, Kari Naylor, Jodi Nunnari; The WD repeat protein, Mdv1p, functions as a molecular adaptor by interacting with Dnm1p and Fis1p during mitochondrial fission . J Cell Biol 5 August 2002; 158 (3): 445–452. doi: https://doi.org/10.1083/jcb.200205031
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
Advertisement