An axonal complex of cell adhesion molecules consisting of Caspr and contactin has been found to be essential for the generation of the paranodal axo-glial junctions flanking the nodes of Ranvier. Here we report that although the extracellular region of Caspr was sufficient for directing it to the paranodes in transgenic mice, retention of the Caspr–contactin complex at the junction depended on the presence of an intact cytoplasmic domain of Caspr. Using immunoelectron microscopy, we found that a Caspr mutant lacking its intracellular domain was often found within the axon instead of the junctional axolemma. We further show that a short sequence in the cytoplasmic domain of Caspr mediated its binding to the cytoskeleton-associated protein 4.1B. Clustering of contactin on the cell surface induced coclustering of Caspr and immobilized protein 4.1B at the plasma membrane. Furthermore, deletion of the protein 4.1B binding site accelerated the internalization of a Caspr–contactin chimera from the cell surface. These results suggest that Caspr serves as a “transmembrane scaffold” that stabilizes the Caspr/contactin adhesion complex at the paranodal junction by connecting it to cytoskeletal components within the axon.
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24 June 2002
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June 24 2002
Retention of a cell adhesion complex at the paranodal junction requires the cytoplasmic region of Caspr
Leora Gollan,
Leora Gollan
1Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
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Helena Sabanay,
Helena Sabanay
1Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
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Sebastian Poliak,
Sebastian Poliak
1Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
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Erik O. Berglund,
Erik O. Berglund
2The Burnham Institute, Neurobiology Program, La Jolla, CA 92037
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Barbara Ranscht,
Barbara Ranscht
2The Burnham Institute, Neurobiology Program, La Jolla, CA 92037
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Elior Peles
Elior Peles
1Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
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Leora Gollan
1Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
Helena Sabanay
1Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
Sebastian Poliak
1Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
Erik O. Berglund
2The Burnham Institute, Neurobiology Program, La Jolla, CA 92037
Barbara Ranscht
2The Burnham Institute, Neurobiology Program, La Jolla, CA 92037
Elior Peles
1Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
Address correspondence to Dr. Elior Peles, Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel. Tel.: 97-28-934-2941. Fax: 97-28-934-4125. E-mail: [email protected]
*
Abbreviations used in this paper: CNS, central nervous system; GST, glutathione-S-transferase; HA, hemagglutinin; PNS, peripheral nervous system.
Received:
March 11 2002
Revision Received:
May 03 2002
Accepted:
May 07 2002
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2002
J Cell Biol (2002) 157 (7): 1247–1256.
Article history
Received:
March 11 2002
Revision Received:
May 03 2002
Accepted:
May 07 2002
Citation
Leora Gollan, Helena Sabanay, Sebastian Poliak, Erik O. Berglund, Barbara Ranscht, Elior Peles; Retention of a cell adhesion complex at the paranodal junction requires the cytoplasmic region of Caspr . J Cell Biol 24 June 2002; 157 (7): 1247–1256. doi: https://doi.org/10.1083/jcb.200203050
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