CHO1 is a kinesin-like protein of the mitotic kinesin-like protein (MKLP)1 subfamily present in central spindles and midbodies in mammalian cells. It is different from other subfamily members in that it contains an extra ∼300 bp in the COOH-terminal tail. Analysis of the chicken genomic sequence showed that heterogeneity is derived from alternative splicing, and exon 18 is expressed in only the CHO1 isoform. CHO1 and its truncated isoform MKLP1 are coexpressed in a single cell. Surprisingly, the sequence encoded by exon 18 possesses a capability to interact with F-actin, suggesting that CHO1 can associate with both microtubule and actin cytoskeletons. Microinjection of exon 18–specific antibodies did not result in any inhibitory effects on karyokinesis and early stages of cytokinesis. However, almost completely separated daughter cells became reunited to form a binulceate cell, suggesting that the exon 18 protein may not have a role in the formation and ingression of the contractile ring in the cortex. Rather, it might be involved directly or indirectly in the membrane events necessary for completion of the terminal phase of cytokinesis.
Skip Nav Destination
Article navigation
4 March 2002
Report|
March 04 2002
CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis
Ryoko Kuriyama,
Ryoko Kuriyama
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
Search for other works by this author on:
Charles Gustus,
Charles Gustus
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
Search for other works by this author on:
Yasuhiko Terada,
Yasuhiko Terada
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
Search for other works by this author on:
Yumi Uetake,
Yumi Uetake
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
Search for other works by this author on:
Jurgita Matuliene
Jurgita Matuliene
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
Search for other works by this author on:
Ryoko Kuriyama
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
Charles Gustus
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
Yasuhiko Terada
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
Yumi Uetake
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
Jurgita Matuliene
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455
Address correspondence to Ryoko Kuriyama, Dept. of Genetics, Cell Biology, and Development, 6-160 Jackson Hall, 321 Church St. SE, University of Minnesota, Minneapolis, MN 55455. Tel.: (612) 624-0471. Fax: (612) 626-6140. E-mail: [email protected]
*
Abbreviations used in this paper: GFP, green fluorescent protein; MKLP, mitotic kinesin-like protein.
Received:
September 24 2001
Revision Received:
January 18 2002
Accepted:
January 18 2002
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2002
J Cell Biol (2002) 156 (5): 783–790.
Article history
Received:
September 24 2001
Revision Received:
January 18 2002
Accepted:
January 18 2002
Citation
Ryoko Kuriyama, Charles Gustus, Yasuhiko Terada, Yumi Uetake, Jurgita Matuliene; CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis . J Cell Biol 4 March 2002; 156 (5): 783–790. doi: https://doi.org/10.1083/jcb.200109090
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
Advertisement