The location of budding proteins is controlled by expression timing.
The genes BUD8 and BUD9 are required for establishment of the bipolar budding pattern in normal diploid cells, in which both poles are specified as potential bud sites. Although the two proteins are similar in structure, they show distinct subcellular localizations, consistent with functions as markers of the distal and proximal poles of daughter cells.Using promoter swap experiments, Schenkman et al. now show that the timing of gene expression determines the localization of Bud8p and Bud9p. The localization of the proteins is probably determined by the direction of the general secretory vesicle traffic at the stage of the cell cycle when the protein is delivered. Although new...
