Rab9 (red) and Rab7 (green) localize to distinct domains on late endosomes.

Barbero et al. have visualized the movement of individual vesicles from late endosomes to the trans Golgi by using GFP-marked Rab9 (page 511). Their results suggest that vesicles rather than tubules are used to deliver mannose 6-phosphate receptors (MPRs) in their return to the Golgi, after these proteins have transported enzymes from the Golgi to prelysosomes.

The detailed localization of the GFP-Rab9 is also telling. Rab GTPases are thought to contribute delivery address information for many trafficking vesicles, but Barbero et al. note that Rab9 is present on the endosome- derived vesicles from budding through docking. This is consistent with the group's earlier experiments suggesting that Rab9 has a function during budding. Perhaps as part of this budding process Rab9 clusters in particular regions, as the authors note that Rab9 and...

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