The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface. This structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil proteins, the golgins, and other peripheral membrane components such as GRASP55 and GRASP65. Here we find that TMP21, p24a, and gp25L, members of the p24 cargo receptor family, are present in complexes with GRASP55 and GRASP65 in vivo. GRASPs interact directly with the cytoplasmic domains of specific p24 cargo receptors depending on their oligomeric state, and mutation of the GRASP binding site in the cytoplasmic tail of one of these, p24a, results in it being transported to the cell surface. These results suggest that one function of the Golgi matrix is to aid efficient retention or sequestration of p24 cargo receptors and other membrane proteins in the Golgi apparatus.
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10 December 2001
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December 10 2001
Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus
Francis A. Barr,
Francis A. Barr
Department of Cell Biology, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
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Christian Preisinger,
Christian Preisinger
Department of Cell Biology, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
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Robert Kopajtich,
Robert Kopajtich
Department of Cell Biology, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
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Roman Körner
Roman Körner
Department of Cell Biology, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
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Francis A. Barr
Department of Cell Biology, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
Christian Preisinger
Department of Cell Biology, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
Robert Kopajtich
Department of Cell Biology, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
Roman Körner
Department of Cell Biology, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
Address correspondence to Francis Barr, Dept. of Cell Biology, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany. Tel.: 49-89-8578-3135. Fax: 49-89-8578-3102. E-mail: [email protected]
*
Abbreviations used in this paper: GFP, green fluorescent protein; GST, glutathione S-transferase.
Received:
August 20 2001
Revision Received:
October 15 2001
Accepted:
October 23 2001
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2001
J Cell Biol (2001) 155 (6): 885–892.
Article history
Received:
August 20 2001
Revision Received:
October 15 2001
Accepted:
October 23 2001
Citation
Francis A. Barr, Christian Preisinger, Robert Kopajtich, Roman Körner; Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus . J Cell Biol 10 December 2001; 155 (6): 885–892. doi: https://doi.org/10.1083/jcb.200108102
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