We examined the mobilities of nucleolar components that act at various steps of the ribosome biogenesis pathway. Fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) analyses demonstrate that factors involved in rRNA transcription (upstream-binding factor [UBF]), processing (nucleolin, fibrillarin, and RNase MRP subunits, Rpp29), and ribosome assembly (B23) exchange rapidly between the nucleoplasm and nucleolus. In contrast, the mobilities of ribosomal subunit proteins (S5, L9) are much slower. Selective inhibition of RNA polymerase I transcription does not prevent the exchanges but influences the rates of exchange differentially for different nucleolar components. These findings suggest that the rapid exchange of nucleolar components between the nucleolus and nucleoplasm may represent a new level of regulation for rRNA synthesis. The different dynamic properties of proteins involved in different steps of ribosome biogenesis imply that the nucleolar association of these proteins is due to their specific functional roles rather than simply their specific nucleolar-targeting events.
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2 April 2001
Article|
April 02 2001
Nucleolar Components Involved in Ribosome Biogenesis Cycle between the Nucleolus and Nucleoplasm in Interphase Cells
Danyang Chen,
Danyang Chen
aDepartment of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611
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Sui Huang
Sui Huang
aDepartment of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611
Search for other works by this author on:
Danyang Chen
aDepartment of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611
Sui Huang
aDepartment of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611
Abbreviations used in this paper: ActD, actinomycin D; FLIP, fluorescence loss in photobleaching; GFP, green fluorescent protein; NOR, nucleolar-organizing region; pol, RNA polymerase; RFI, relative fluorescence intensity; TBP, TATA-binding protein; UBF, upstream-binding factor.
Received:
November 16 2000
Revision Requested:
February 13 2001
Accepted:
February 14 2001
Online ISSN: 1540-8140
Print ISSN: 0021-9525
© 2001 The Rockefeller University Press
2001
The Rockefeller University Press
J Cell Biol (2001) 153 (1): 169–176.
Article history
Received:
November 16 2000
Revision Requested:
February 13 2001
Accepted:
February 14 2001
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Citation
Danyang Chen, Sui Huang; Nucleolar Components Involved in Ribosome Biogenesis Cycle between the Nucleolus and Nucleoplasm in Interphase Cells. J Cell Biol 2 April 2001; 153 (1): 169–176. doi: https://doi.org/10.1083/jcb.153.1.169
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