Cdc24p, the GDP/GTP exchange factor for the regulator of actin cytoskeleton Cdc42p, localizes to sites of polarized growth. Here we show that Cdc24p shuttles in and out of the yeast nucleus during vegetative growth. Far1p is necessary and sufficient for nuclear accumulation of Cdc24p, suggesting that its nuclear import occurs via an association with Far1p. Nuclear export is triggered either by entry into the cell cycle or by mating pheromone. As Far1p is degraded upon entry into the cell cycle, cell cycle–dependent export of Cdc24p occurs in the absence of Far1p, whereas during mating similar export kinetics indicate that a Cdc24p–Far1p complex is exported. Our results suggest that the nucleus serves as a store of preformed Cdc24p–Far1p complex which is required for chemotropism.
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20 March 2000
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March 20 2000
Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p
Aljoscha Nern,
Aljoscha Nern
aDivision of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, United Kingdom
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Robert A. Arkowitz
Robert A. Arkowitz
aDivision of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, United Kingdom
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Aljoscha Nern
aDivision of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, United Kingdom
Robert A. Arkowitz
aDivision of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, United Kingdom
Received:
December 22 1999
Revision Requested:
February 02 2000
Accepted:
February 08 2000
Online ISSN: 1540-8140
Print ISSN: 0021-9525
© 2000 The Rockefeller University Press
2000
The Rockefeller University Press
J Cell Biol (2000) 148 (6): 1115–1122.
Article history
Received:
December 22 1999
Revision Requested:
February 02 2000
Accepted:
February 08 2000
Citation
Aljoscha Nern, Robert A. Arkowitz; Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p. J Cell Biol 20 March 2000; 148 (6): 1115–1122. doi: https://doi.org/10.1083/jcb.148.6.1115
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