Syntaxin 13 Mediates Cycling of Plasma Membrane Proteins via Tubulovesicular Recycling Endosomes

Prekeris, Rytis; Klumperman, Judith; Chen, Yu A.; Scheller, Richard H.

doi:10.1083/jcb.143.4.957

Article navigation

Article| November 16 1998

Syntaxin 13 Mediates Cycling of Plasma Membrane Proteins via Tubulovesicular Recycling Endosomes

Rytis Prekeris,

Rytis Prekeris

*Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5428; and ‡Medical School, University of Utrecht, Institute for Biomembranes, 3584CX Utrecht, The Netherlands

Search for other works by this author on:

This Site

PubMed

Google Scholar

Judith Klumperman,

Judith Klumperman

*Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5428; and ‡Medical School, University of Utrecht, Institute for Biomembranes, 3584CX Utrecht, The Netherlands

Search for other works by this author on:

This Site

PubMed

Google Scholar

Yu A. Chen,

Yu A. Chen

*Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5428; and ‡Medical School, University of Utrecht, Institute for Biomembranes, 3584CX Utrecht, The Netherlands

Search for other works by this author on:

This Site

PubMed

Google Scholar

Richard H. Scheller

*Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5428; and ‡Medical School, University of Utrecht, Institute for Biomembranes, 3584CX Utrecht, The Netherlands

Search for other works by this author on:

This Site

PubMed

Google Scholar

Author and Article Information

Rytis Prekeris

*Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5428; and ‡Medical School, University of Utrecht, Institute for Biomembranes, 3584CX Utrecht, The Netherlands

Judith Klumperman

*Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5428; and ‡Medical School, University of Utrecht, Institute for Biomembranes, 3584CX Utrecht, The Netherlands

Yu A. Chen

*Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5428; and ‡Medical School, University of Utrecht, Institute for Biomembranes, 3584CX Utrecht, The Netherlands

Richard H. Scheller

*Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5428; and ‡Medical School, University of Utrecht, Institute for Biomembranes, 3584CX Utrecht, The Netherlands

Address correspondence to R.H. Scheller, Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305-5428. Tel.: (650) 723-9075. Fax: (650) 725-4436. E-mail: [email protected]

We would like to acknowledge D. Foletti (Stanford University, Stanford, CA) for his help in culturing embryonic hippocampal neurons. We also thank M. Steegmaier (Stanford University) for assistance in hybridoma culturing and R. Winant of the Stanford PAN facility for amino acid sequencing. We thank W. Stoorvogel (University of Utrecht, Utrecht, The Netherlands) for providing useful suggestions on the recycling assay and the critical reading of the manuscript. We would also like to thank C. Hazuka for the critical reading of the manuscript. M. Niekerk, R. Scriwanek, and T. van Rijn (all from University of Utrecht) are acknowledged for the preparation of the electronmicrographs. V. Oorschot (University of Utrecht) is gratefully acknowledged for the preparation of ultrathin cryosections.

Received: August 05 1998

Revision Received: October 07 1998

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1998

J Cell Biol (1998) 143 (4): 957–971.

https://doi.org/10.1083/jcb.143.4.957

Endocytosis-mediated recycling of plasma membrane is a critical vesicle trafficking step important in diverse biological processes. The membrane trafficking decisions and sorting events take place in a series of heterogeneous and highly dynamic organelles, the endosomes. Syntaxin 13, a recently discovered member of the syntaxin family, has been suggested to play a role in mediating endosomal trafficking. To better understand the function of syntaxin 13 we examined its intracellular distribution in nonpolarized cells. By confocal immunofluorescence and electron microscopy, syntaxin 13 is primarily found in tubular early and recycling endosomes, where it colocalizes with transferrin receptor. Additional labeling is also present in endosomal vacuoles, where it is often found in clathrin-coated membrane areas. Furthermore, anti-syntaxin 13 antibody inhibits transferrin receptor recycling in permeabilized PC12 cells. Immunoprecipitation of syntaxin 13 revealed that, in Triton X-100 extracts, syntaxin 13 is present in a complex(es) comprised of βSNAP, VAMP 2/3, and SNAP-25. This complex(es) binds exogenously added αSNAP and NSF and dissociates in the presence of ATP, but not ATPγS. These results support a role for syntaxin 13 in membrane fusion events during the recycling of plasma membrane proteins.

1998

You do not currently have access to this content.

Don't already have an account? Register

You could not be signed in. Please check your email address / username and password and try again.

Syntaxin 13 Mediates Cycling of Plasma Membrane Proteins via Tubulovesicular Recycling Endosomes

Sign in

Client Account

Sign in via your Institution

Suggested Content

Email alerts

This Feature Is Available To Subscribers Only