We examined the heterochromatic binding of GAGA factor and proliferation disrupter (Prod) proteins during the cell cycle in Drosophila melanogaster and sibling species. GAGA factor binding to the brownDominant AG-rich satellite sequence insertion was seen at metaphase, however, no binding of GAGA factor to AG-rich sequences was observed at interphase in polytene or diploid nuclei. Comparable mitosis-specific binding was found for Prod protein to its target satellite in pericentric heterochromatin. At interphase, these proteins bind numerous dispersed sites in euchromatin, indicating that they move from euchromatin to heterochromatin and back every cell cycle. The presence of Prod in heterochromatin for a longer portion of the cell cycle than GAGA factor suggests that they cycle between euchromatin and heterochromatin independently. We propose that movement of GAGA factor and Prod from high affinity sites in euchromatin occurs upon condensation of metaphase chromosomes. Upon decondensation, GAGA factor and Prod shift from low affinity sites within satellite DNA back to euchromatic sites as a self-assembly process.
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23 March 1998
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March 23 1998
Changes in Chromosomal Localization of Heterochromatin-binding Proteins during the Cell Cycle in Drosophila
J. Suso Platero,
J. Suso Platero
Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024
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Amy K. Csink,
Amy K. Csink
Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024
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Adrian Quintanilla,
Adrian Quintanilla
Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024
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Steven Henikoff
Steven Henikoff
Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024
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J. Suso Platero
Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024
Amy K. Csink
Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024
Adrian Quintanilla
Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024
Steven Henikoff
Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024
Address all correspondence to Steven Henikoff, Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024. Tel.: (206) 667-4515. Fax: (206) 667-5889. E-mail: [email protected]
Received:
October 21 1997
Revision Received:
January 22 1998
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1998
J Cell Biol (1998) 140 (6): 1297–1306.
Article history
Received:
October 21 1997
Revision Received:
January 22 1998
Citation
J. Suso Platero, Amy K. Csink, Adrian Quintanilla, Steven Henikoff; Changes in Chromosomal Localization of Heterochromatin-binding Proteins during the Cell Cycle in Drosophila . J Cell Biol 23 March 1998; 140 (6): 1297–1306. doi: https://doi.org/10.1083/jcb.140.6.1297
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