gp25L/emp24/p24 Protein Family Members of the cis-Golgi Network Bind Both COP I and II Coatomer

We thank the following colleagues for kindly sharing reagents, even before their publication: J. Rothman (Sloan-Kettering, NY) and F. Wieland (University of Heidelberg, Heidelberg, Germany) for gifts of p24 and p23 antibodies; T. Kreis (University of Geneva, Geneva, Switzerland) and C. Harter (University of Heidelberg ) for COP I reagents; H.-P. Hauri (University of Basel, Basel, Switzerland) and J. Saraste (University of Bergen, Bergen, Norway) for sharing p53 and p58 antibodies; H.-D. Söling (University of Göttingen, Göttingen, Germany), for the KDEL receptor antibody; N. Hui (Imperial Cancer Research Fund, London, England) for syntaxin 5 antibodies and J. Ostermann (Vanderbilt University School of Medicine, Nashville, TN) for purified coatomer I. Also, we wish to acknowledge F. Parlati (Genetics Group, Biotechnology Research Institute, Montreal, Canada) for help in cloning; B. Storrie (Blacksburg) and M. Otter (Cell Biology Programme, European Molecular Biology Laboratory [EMBL], Heidelberg, Germany), for critically reading the manuscript. EST's were obtained from the Institute for Genomic Research database TIGR and from GenBank. We would also like to thank the support facilities at EMBL along with A. Bell, P. Cameron, and the Sheldon Biotechnology center at McGill for antibody production, peptide sequencing, and DNA sequencing.

This work was supported by a McGill major Hydro-Québec fellowship to M. Dominguez, a Deutsche Forschungsgemeinschaft to J. Füllekrug, a Swiss National Science Fundation grant (31-43366-95) and Jules Thorn Charitable Overseas Trust to J.-P. Paccaud, and the Medical Research Council of Canada and Glaxo Wellcome Inc. to J.J.M. Bergeron.

M. Dominguez and K. Dejgaard contributed equally to this work.

Address all correspondence to T. Nilsson, Cell Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69012 Heidelberg, Germany. Tel.: (49) 622-138-7294. Fax: (49) 622-138-7512. E-mail: [email protected]

1. Abbreviations used in this paper: CGN, cis-Golgi network; COP, coat proteins; ECL, enhanced chemiluminescence; endo H, endo glycosidase H; ERGIC, ER-to-GOLGI intermediate compartment; GalNAc-T1, N-acetylgalactosylaminyltransferase I; GalT, β1,4-galactosyltransferase; Man II, α1,2 mannosidase II; MLP, multiple large platform; NAGT I, N-acetylglucosaminyltransferase I; RT, reverse transcriptase; Sialyl, α2,6-sialytransferase; UDP-GT, UDP-glucuronyltransferase.