We have used an insertional mutagenesis/ gene tagging technique to generate new Chlamydomonas reinhardtii mutants that are defective in assembly of the outer dynein arm. Among 39 insertional oda mutants characterized, two are alleles of the previously uncloned ODA3 gene, one is an allele of the uncloned ODA10 gene, and one represents a novel ODA gene (termed ODA12). ODA3 is of particular interest because it is essential for assembly of both the outer dynein arm and the outer dynein arm docking complex (ODA-DC) onto flagellar doublet microtubules (Takada, S., and R. Kamiya. 1994. J. Cell Biol. 126:737– 745). Beginning with the inserted DNA as a tag, the ODA3 gene and a full-length cDNA were cloned. The cloned gene rescues the phenotype of oda3 mutants. The cDNA sequence predicts a novel 83.4-kD protein with extensive coiled-coil domains. The ODA-DC contains three polypeptides; direct amino acid sequencing indicates that the largest of these polypeptides corresponds to ODA3. This protein is likely to have an important role in the precise positioning of the outer dynein arms on the flagellar axoneme.
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2 June 1997
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June 02 1997
The Chlamydomonas reinhardtii ODA3 Gene Encodes a Protein of the Outer Dynein Arm Docking Complex
Anthony Koutoulis,
Anthony Koutoulis
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
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Gregory J. Pazour,
Gregory J. Pazour
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
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Curtis G. Wilkerson,
Curtis G. Wilkerson
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
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Kazuo Inaba,
Kazuo Inaba
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
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Hong Sheng,
Hong Sheng
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
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Saeko Takada,
Saeko Takada
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
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George B. Witman
George B. Witman
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
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Anthony Koutoulis
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
Gregory J. Pazour
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
Curtis G. Wilkerson
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
Kazuo Inaba
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
Hong Sheng
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
Saeko Takada
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
George B. Witman
*Department of Plant Science, The University of Tasmania, Hobart TAS 7001 Australia; ‡Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545; §Misaki Marine Biological Station, The University of Tokyo, Kanagawa 238-02 Japan; and ‖Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113 Japan
1. Abbreviations used in this paper: DHC, dynein heavy chain; IC, dynein intermediate chain; LC, dynein light chain; ODA-DC, outer dynein arm docking complex; RFLP, restriction fragment length polymorphism.
2. To conform to recommendations for a standard genetic nomenclature in Chlamydomonas (Dutcher, 1995), genetic loci and the wild-type allele for those loci are denoted using uppercase italic letters, followed by a number when there is more than one locus with the same name (e.g., ODA3); mutant alleles are designated in lowercase italic (e.g., oda3); different mutant alleles at the same locus are distinguished by a number separated from the locus designation by a hyphen (e.g., oda3-4); phenotypes are indicated by an uppercase letter followed by lowercase letters and a plus or minus (e.g., Oda−), and gene products are designated using uppercase roman letters (e.g., ODA3).
Please address all correspondence to George B. Witman, Worcester Foundation for Biomedical Research, 222 Maple Avenue, Shrewsbury, MA 01545. Tel.: (508) 842-8921 ext. 344. Fax: (508) 842-3915.
Received:
February 20 1997
Revision Received:
April 04 1997
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1997
J Cell Biol (1997) 137 (5): 1069–1080.
Article history
Received:
February 20 1997
Revision Received:
April 04 1997
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Citation
Anthony Koutoulis, Gregory J. Pazour, Curtis G. Wilkerson, Kazuo Inaba, Hong Sheng, Saeko Takada, George B. Witman; The Chlamydomonas reinhardtii ODA3 Gene Encodes a Protein of the Outer Dynein Arm Docking Complex. J Cell Biol 2 June 1997; 137 (5): 1069–1080. doi: https://doi.org/10.1083/jcb.137.5.1069
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