ERS-24, a Mammalian v-SNARE Implicated in Vesicle Traffic between the ER and the Golgi

Paek, Inbok; Orci, Lelio; Ravazzola, Mariella; Erdjument-Bromage, Hediye; Amherdt, Mylene; Tempst, Paul; Söllner, Thomas H.; Rothman, James E.

doi:10.1083/jcb.137.5.1017

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Article| June 02 1997

ERS-24, a Mammalian v-SNARE Implicated in Vesicle Traffic between the ER and the Golgi

Inbok Paek,

Inbok Paek

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

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Lelio Orci,

Lelio Orci

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

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Mariella Ravazzola,

Mariella Ravazzola

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

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Hediye Erdjument-Bromage,

Hediye Erdjument-Bromage

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

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Mylene Amherdt,

Mylene Amherdt

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

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Paul Tempst,

Paul Tempst

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

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Thomas H. Söllner,

Thomas H. Söllner

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

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James E. Rothman

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

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Author and Article Information

Inbok Paek

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

Lelio Orci

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

Mariella Ravazzola

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

Hediye Erdjument-Bromage

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

Mylene Amherdt

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

Paul Tempst

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

Thomas H. Söllner

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

James E. Rothman

*Cellular Biochemistry and Biophysics Program, ‡Molecular Biology Program, §Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021; and ‖Institute of Histology and Embryology, Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland

1. Abbreviations used in this paper: GST, glutathione-S-transferase; NSF, N-ethylmaleimide–sensitive factor; SNAP, soluble NSF attachment protein; SNARE, SNAP receptor.

Please address all correspondence to James E. Rothman, Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, Box 251, New York 10021. Tel.: (212) 6398598. Fax: (212) 717-3604.

Received: November 01 1996

Revision Received: April 03 1997

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1997

J Cell Biol (1997) 137 (5): 1017–1028.

https://doi.org/10.1083/jcb.137.5.1017

We report the identification and characterization of ERS-24 (Endoplasmic Reticulum SNARE of 24 kD), a new mammalian v-SNARE implicated in vesicular transport between the ER and the Golgi. ERS24 is incorporated into 20S docking and fusion particles and disassembles from this complex in an ATP-dependent manner. ERS-24 has significant sequence homology to Sec22p, a v-SNARE in Saccharomyces cerevisiae required for transport between the ER and the Golgi. ERS-24 is localized to the ER and to the Golgi, and it is enriched in transport vesicles associated with these organelles.

1997

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