Receptor protein tyrosine phosphatase β (RPTPβ) is expressed as soluble and receptor forms with common extracellular regions consisting of a carbonic anhydrase domain (C), a fibronectin type III repeat (F), and a unique region called S. We showed previously that a recombinant Fc fusion protein with the C domain (βC) binds to contactin and supports neuronal adhesion and neurite growth. As a substrate, βCFS was less effective in supporting cell adhesion, but it was a more effective promoter of neurite outgrowth than βCF. βS had no effect by itself, but it potentiated neurite growth when mixed with βCF. Neurite outgrowth induced by βCFS was inhibited by antibodies against Nr-CAM and contactin, and these cell adhesion molecules formed a complex that bound βCFS. NIH3T3 cells transfected to express βCFS on their surfaces induced neuronal differentiation in culture. These results suggest that binding of glial RPTPβ to the contactin/Nr-CAM complex is important for neurite growth and neuronal differentiation.
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24 February 1997
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February 24 1997
Induction of Neurite Outgrowth through Contactin and Nr-CAM by Extracellular Regions of Glial Receptor Tyrosine Phosphatase β
Takeshi Sakurai,
Takeshi Sakurai
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
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Marc Lustig,
Marc Lustig
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
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Moshe Nativ,
Moshe Nativ
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
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John J. Hemperly,
John J. Hemperly
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
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Joseph Schlessinger,
Joseph Schlessinger
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
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Elior Peles,
Elior Peles
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
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Martin Grumet
Martin Grumet
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
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Takeshi Sakurai
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
Marc Lustig
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
Moshe Nativ
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
John J. Hemperly
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
Joseph Schlessinger
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
Elior Peles
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
Martin Grumet
*Department of Pharmacology, New York University Medical Center, New York 10016; ‡SUGEN, Inc., Redwood City, California 94063-4720; and §Becton Dickinson Research Center, Becton Dickinson and Company, P.O. Box 12016, Research Triangle Park, North Carolina 27709-2016
Please address all correspondence to Martin Grumet, Department of Pharmacology, New York University Medical Center, New York, NY 10016. Tel.: (212) 263–7126; Fax.: (212) 263-7133; E-mail: [email protected]
Received:
July 19 1996
Revision Received:
October 25 1996
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1997
J Cell Biol (1997) 136 (4): 907–918.
Article history
Received:
July 19 1996
Revision Received:
October 25 1996
Citation
Takeshi Sakurai, Marc Lustig, Moshe Nativ, John J. Hemperly, Joseph Schlessinger, Elior Peles, Martin Grumet; Induction of Neurite Outgrowth through Contactin and Nr-CAM by Extracellular Regions of Glial Receptor Tyrosine Phosphatase β. J Cell Biol 24 February 1997; 136 (4): 907–918. doi: https://doi.org/10.1083/jcb.136.4.907
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