The goal of this research is to identify and characterize the protein machinery that functions in the intracellular translocation and assembly of peroxisomal proteins in Saccharomyces cerevisiae. Several genes encoding proteins that are essential for this process have been identified previously by Kunau and collaborators, but the mutant collection was incomplete. We have devised a positive selection procedure that identifies new mutants lacking peroxisomes or peroxisomal function. Immunofluorescence procedures for yeast were simplified so that these mutants could be rapidly and efficiently screened for those in which peroxisome biogenesis is impaired. With these tools, we have identified four complementation groups of peroxisome biogenesis mutants, and one group that appears to express reduced amounts of peroxisomal proteins. Two of our mutants lack recognizable peroxisomes, although they might contain peroxisomal membrane ghosts like those found in Zellweger syndrome. Two are selectively defective in packaging peroxisomal proteins and moreover show striking intracellular clustering of the peroxisomes. The distribution of mutants among complementation groups implies that the collection of peroxisome biogenesis mutants is still incomplete. With the procedures described, it should prove straightforward to isolate mutants from additional complementation groups.
Skip Nav Destination
Article navigation
1 December 1993
Article|
December 01 1993
Novel peroxisome clustering mutants and peroxisome biogenesis mutants of Saccharomyces cerevisiae.
J W Zhang,
J W Zhang
Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York 10029.
Search for other works by this author on:
Y Han,
Y Han
Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York 10029.
Search for other works by this author on:
P B Lazarow
P B Lazarow
Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York 10029.
Search for other works by this author on:
J W Zhang
Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York 10029.
Y Han
Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York 10029.
P B Lazarow
Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York 10029.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1993) 123 (5): 1133–1147.
Citation
J W Zhang, Y Han, P B Lazarow; Novel peroxisome clustering mutants and peroxisome biogenesis mutants of Saccharomyces cerevisiae.. J Cell Biol 1 December 1993; 123 (5): 1133–1147. doi: https://doi.org/10.1083/jcb.123.5.1133
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
Advertisement