We have previously demonstrated that the preendosomal compartment in addition to clathrin-coated vesicles, comprises distinct nonclathrin coated endocytic vesicles mediating clathrin-independent endocytosis (Hansen, S. H., K. Sandvig, and B. van Deurs. 1991. J. Cell Biol. 113:731-741). Using K+ depletion in HEp-2 cells to block clathrin-dependent but not clathrin-independent endocytosis, we have now traced the intracellular routing of these nonclathrin coated vesicles to see whether molecules internalized by clathrin-independent endocytosis are delivered to a unique compartment or whether they reach the same early and late endosomes as encountered by molecules internalized with high efficiency through clathrin-coated pits and vesicles. We find that Con A-gold internalized by clathrin-independent endocytosis is delivered to endosomes containing transferrin receptors. After incubation of K(+)-depleted cells with Con A-gold for 15 min, approximately 75% of Con A-gold in endosomes is colocalized with transferrin receptors. Endosomes containing only Con A-gold may be accounted for either by depletion of existing endosomes for transferrin receptors or by de novo generation of endosomes. Cationized gold and BSA-gold internalized in K(+)-depleted cells are also delivered to endosomes containing transferrin receptors. h-lamp-1-enriched compartments are only reached occasionally within 30 min in K(+)-depleted as well as in control cells. Thus, preendosomal vesicles generated by clathrin-independent endocytosis do not fuse to any marked degree with late endocytic compartments. These data show that in HEp-2 cells, molecules endocytosed without clathrin are delivered to the same endosomes as reached by transferrin receptors internalized through clathrin-coated pits.
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1 October 1993
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October 01 1993
Molecules internalized by clathrin-independent endocytosis are delivered to endosomes containing transferrin receptors.
S H Hansen,
S H Hansen
Department of Anatomy, Panum Institute, University of Copenhagen, Denmark.
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K Sandvig,
K Sandvig
Department of Anatomy, Panum Institute, University of Copenhagen, Denmark.
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B van Deurs
B van Deurs
Department of Anatomy, Panum Institute, University of Copenhagen, Denmark.
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S H Hansen
Department of Anatomy, Panum Institute, University of Copenhagen, Denmark.
K Sandvig
Department of Anatomy, Panum Institute, University of Copenhagen, Denmark.
B van Deurs
Department of Anatomy, Panum Institute, University of Copenhagen, Denmark.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1993) 123 (1): 89–97.
Citation
S H Hansen, K Sandvig, B van Deurs; Molecules internalized by clathrin-independent endocytosis are delivered to endosomes containing transferrin receptors.. J Cell Biol 1 October 1993; 123 (1): 89–97. doi: https://doi.org/10.1083/jcb.123.1.89
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