In contrast to many other peroxisomal proteins catalase A contains at least two peroxisomal targeting signals each sufficient to direct reporter proteins to peroxisomes. One of them resides at the extreme carboxy terminus constituting a new variant of this signal, -SSNSKF, not active in monkey kidney cells (Gould, S. J., G. A. Keller, N. Hosken, J. Wilkinson, and S. Subramani 1989. J. Cell Biol. 108:1657-1664). However, this signal is completely dispensable for import of catalase A itself. In its amino-terminal third this protein contains another peroxisomal targeting signal sufficient to direct reporter proteins into microbodies. This internal signal depends on the context. The nature of this targeting signal might be a short defined sequence or a structural feature recognized by import factors. In addition, we have demonstrated that the carboxy-terminal seven amino acids of citrate synthase of Saccharomyces cerevisiae encoded by CIT2 and containing the canonical -SKL represents a targeting signal sufficient to direct reporter proteins to peroxisomes.
Article|
February 01 1993
Two independent peroxisomal targeting signals in catalase A of Saccharomyces cerevisiae.
F Kragler,
F Kragler
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
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A Langeder,
A Langeder
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
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J Raupachova,
J Raupachova
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
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M Binder,
M Binder
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
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A Hartig
A Hartig
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
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F Kragler
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
A Langeder
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
J Raupachova
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
M Binder
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
A Hartig
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1993) 120 (3): 665–673.
Citation
F Kragler, A Langeder, J Raupachova, M Binder, A Hartig; Two independent peroxisomal targeting signals in catalase A of Saccharomyces cerevisiae.. J Cell Biol 1 February 1993; 120 (3): 665–673. doi: https://doi.org/10.1083/jcb.120.3.665
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