Chondrocytes at different stages of cellular differentiation were isolated from the tarsal element (immature chondrocytes) and zones 2 and 3 (mature chondrocytes) of 12-d chick embryo tibiotarsus. The chondrocytes from the two sources differed in their cell morphologies, growth rate and production of type X collagen. In 24 h, zone 2 and 3 chondrocytes synthesized 800 times more type X collagen than tarsal chondrocytes. The effect of exogenous CaCl2 (5 and 10 mM) on the synthesis of type X collagen by both mature and immature chondrocytes was tested. After a 72-h incubation of zone 2 and 3 chondrocytes with CaCl2 type X collagen increased 8-fold with 5 mM and 10-fold with 10 mM Ca2+. [3H]Proline incorporation into culture medium and matrix macromolecules increased 11 and 32% with 5 and 10 mM CaCl2, respectively. Type II collagen synthesis was not affected by elevated extracellular Ca2+ during this 72-h period. Similar studies with tarsal chondrocytes demonstrated a time- and dose-dependent response to CaCl2 with type X collagen levels reaching a 4-fold and 15-fold increase over controls with 5 and 10 mM Ca2+, respectively, at 48 h. Elevated extracellular Ca2+ had no effect on cell proliferation. These observations offer the first direct evidence of the induction of type X collagen synthesis with elevated extracellular Ca2+.
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15 November 1991
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November 15 1991
Elevated extracellular calcium concentrations induce type X collagen synthesis in chondrocyte cultures.
D K Bonen,
D K Bonen
Department of Biochemistry, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
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T M Schmid
T M Schmid
Department of Biochemistry, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
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D K Bonen
Department of Biochemistry, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
T M Schmid
Department of Biochemistry, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1991) 115 (4): 1171–1178.
Citation
D K Bonen, T M Schmid; Elevated extracellular calcium concentrations induce type X collagen synthesis in chondrocyte cultures.. J Cell Biol 15 November 1991; 115 (4): 1171–1178. doi: https://doi.org/10.1083/jcb.115.4.1171
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