Granule membrane protein-140 (GMP-140) is an inducible receptor for myeloid leukocytes on activated platelets and endothelium. Like other selectins, GMP-140 recognizes specific oligosaccharide ligands. However, prior data on the nature of these ligands are contradictory. We investigated the structural features required for ligand interaction with GMP-140 using purified GMP-140, cells naturally expressing specific oligosaccharides, and cells expressing cloned glycosyltransferases. Like the related selectin endothelial leukocyte adhesion molecule-1 (ELAM-1), GMP-140 recognizes alpha(2-3)sialylated, alpha(1-3)fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells, including the sequence Neu5Ac alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNac beta-R (sialyl Lewis x). Recognition requires sialic acid, because cells expressing large amounts of Lewis x, but not sialyl Lewis x, do not interact with GMP-140. Although sialyl Lewis x is expressed by both myeloid HL-60 cells and CHO cells transfected with an alpha 1-3/4 fucosyltransferase, GMP-140 binds with significantly higher affinity to HL-60 cells. Thus, the sialyl Lewis x tetrasaccharide may require additional structural modifications or specific presentations in order for leukocytes in flowing blood to interact rapidly and with high affinity to GMP-140 on activated platelets or endothelium.
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15 October 1991
Article|
October 15 1991
The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells.
Q Zhou,
Q Zhou
Department of Biochemistry, University of Georgia, Athens 30602.
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K L Moore,
K L Moore
Department of Biochemistry, University of Georgia, Athens 30602.
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D F Smith,
D F Smith
Department of Biochemistry, University of Georgia, Athens 30602.
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A Varki,
A Varki
Department of Biochemistry, University of Georgia, Athens 30602.
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R P McEver,
R P McEver
Department of Biochemistry, University of Georgia, Athens 30602.
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R D Cummings
R D Cummings
Department of Biochemistry, University of Georgia, Athens 30602.
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Q Zhou
Department of Biochemistry, University of Georgia, Athens 30602.
K L Moore
Department of Biochemistry, University of Georgia, Athens 30602.
D F Smith
Department of Biochemistry, University of Georgia, Athens 30602.
A Varki
Department of Biochemistry, University of Georgia, Athens 30602.
R P McEver
Department of Biochemistry, University of Georgia, Athens 30602.
R D Cummings
Department of Biochemistry, University of Georgia, Athens 30602.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1991) 115 (2): 557–564.
Citation
Q Zhou, K L Moore, D F Smith, A Varki, R P McEver, R D Cummings; The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells.. J Cell Biol 15 October 1991; 115 (2): 557–564. doi: https://doi.org/10.1083/jcb.115.2.557
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