Saccharomyces cerevisiae sec7 mutants exhibit pleiotropic deficiencies in the transit of proteins through the Golgi apparatus, and elaborate an array of Golgi apparatus-like cisternae at a restrictive growth temperature (37 degrees C). The SEC7 gene encodes an essential high-molecular weight protein (227 kD) that is phosphorylated in vivo. In cell lysates, Sec7 protein (Sec7p) is recovered in both sedimentable and soluble fractions. A punctate immunofluorescent pattern of Sec7p-associated structures seen in SEC cells coalesces in sec14 mutant yeast that accumulate exaggerated Golgi cisternae at 37 degrees C. Sec7p may function as a peripheral membrane protein that cycles between a soluble, cytosolic pool and a sedimentable, membrane-associated complex for its essential role in vesicular traffic through the Golgi apparatus. The transmembrane Kex2 protease, which processes precursors of secreted peptides within the yeast secretory pathway, is also localized by indirect immunofluorescence to multiple structures in the yeast cell (Redding, K., and R. Fuller, manuscript submitted for publication). In double-immunofluorescence labeling experiments, significant colocalization of Sec7 and Kex2 proteins was found. Colocalization of the two antigens, one implicated in protein transport through the Golgi apparatus and the other in processing within a late Golgi compartment, supports the conclusion that we have visualized the yeast Golgi apparatus.
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1 January 1991
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January 01 1991
Localization of components involved in protein transport and processing through the yeast Golgi apparatus.
A Franzusoff,
A Franzusoff
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262.
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K Redding,
K Redding
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262.
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J Crosby,
J Crosby
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262.
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R S Fuller,
R S Fuller
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262.
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R Schekman
R Schekman
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262.
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A Franzusoff
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262.
K Redding
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262.
J Crosby
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262.
R S Fuller
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262.
R Schekman
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1991) 112 (1): 27–37.
Citation
A Franzusoff, K Redding, J Crosby, R S Fuller, R Schekman; Localization of components involved in protein transport and processing through the yeast Golgi apparatus.. J Cell Biol 1 January 1991; 112 (1): 27–37. doi: https://doi.org/10.1083/jcb.112.1.27
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