We have previously identified three distinctive amino acid sequences from type IV collagen which specifically bound to heparin and also inhibited the binding of heparin to intact type IV collagen. One of these chemically synthesized domains, peptide Hep-I, has the sequence TAGSCLRKFSTM and originates from the a1(noncollagenous [NC1]) chain of type IV collagen (Koliakos, G. G., K. K. Koliakos, L. T. Furcht, L. A. Reger, and E. C. Tsilibary. 1989. J. Biol. Chem. 264:2313-2323). We describe in this report that this same peptide also bound to intact type IV collagen in solid-phase assays, in a dose-dependent and specific manner. Interactions between peptide Hep-I and type IV collagen in solution resulted in inhibition of the assembly process of this basement membrane glycoprotein. Therefore, peptide Hep-I should represent a major recognition site in type IV collagen when this protein polymerizes to form a network. In addition, solid phase-immobilized peptide Hep-I was able to promote the adhesion and spreading of bovine aortic endothelial cells. When present in solution, peptide Hep-I competed for the binding of these cells to type IV collagen- and NC1 domain-coated substrata in a dose-dependent manner. Furthermore, radiolabeled peptide Hep-I in solution also bound to endothelial cells in a dose-dependent and specific manner. The binding of radiolabeled Hep-I to endothelial cells could be inhibited by an excess of unlabeled peptide. Finally, in the presence of heparin or chondroitin/dermatan sulfate glycosaminoglycan side chains, the binding of endothelial cells to peptide Hep-I and NC1 domain-coated substrates was also inhibited. We conclude that peptide Hep-I should have a number of functions. The role of this type IV collagen-derived sequence in such diverse phenomena as self-association, heparin binding and cell binding and adhesion makes Hep-I a crucial domain involved in the determination of basement membrane ultrastructure and cellular interactions with type IV collagen-containing matrices.
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1 October 1990
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October 01 1990
Identification of a multifunctional, cell-binding peptide sequence from the a1(NC1) of type IV collagen.
E C Tsilibary,
E C Tsilibary
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
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L A Reger,
L A Reger
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
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A M Vogel,
A M Vogel
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
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G G Koliakos,
G G Koliakos
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
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S S Anderson,
S S Anderson
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
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A S Charonis,
A S Charonis
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
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J N Alegre,
J N Alegre
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
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L T Furcht
L T Furcht
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
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E C Tsilibary
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
L A Reger
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
A M Vogel
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
G G Koliakos
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
S S Anderson
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
A S Charonis
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
J N Alegre
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
L T Furcht
University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1990) 111 (4): 1583–1591.
Citation
E C Tsilibary, L A Reger, A M Vogel, G G Koliakos, S S Anderson, A S Charonis, J N Alegre, L T Furcht; Identification of a multifunctional, cell-binding peptide sequence from the a1(NC1) of type IV collagen.. J Cell Biol 1 October 1990; 111 (4): 1583–1591. doi: https://doi.org/10.1083/jcb.111.4.1583
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