We have used autoantibodies to probe the function of three human centromere proteins in mitosis. These antibodies recognize three human polypeptides in immunoblots: CENP-A (17 kD), CENP-B (80 kD), and CENP-C (140 kD). Purified anticentromere antibodies (ACA-IgG) disrupt mitosis when introduced into tissue culture cells during interphase. We have identified two execution points for antibody inhibition. Antibodies injected into the nucleus greater than or equal to 3 h before mitosis prevent the chromosomes from undergoing normal prometaphase movements in the subsequent mitosis. Antibodies injected in the nucleus during late G2 cause cells to arrest in metaphase. Surprisingly, antibodies introduced subsequent to the beginning of prophase do not block mitosis. These results suggest that the CENP antigens are involved in two essential interphase events that are required for centromere action in mitosis. These may include centromere assembly coordinate with the replication of alpha-satellite DNA at the end of S phase and the structural maturation of the kinetochore that begins at prophase.
Skip Nav Destination
Article navigation
1 October 1990
Article|
October 01 1990
Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis.
R L Bernat,
R L Bernat
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Search for other works by this author on:
G G Borisy,
G G Borisy
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Search for other works by this author on:
N F Rothfield,
N F Rothfield
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Search for other works by this author on:
W C Earnshaw
W C Earnshaw
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Search for other works by this author on:
R L Bernat
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
G G Borisy
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
N F Rothfield
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
W C Earnshaw
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1990) 111 (4): 1519–1533.
Citation
R L Bernat, G G Borisy, N F Rothfield, W C Earnshaw; Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis.. J Cell Biol 1 October 1990; 111 (4): 1519–1533. doi: https://doi.org/10.1083/jcb.111.4.1519
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
Advertisement