Figure 1. Models for ChiMERA rescue of ERMES depletion. (A) Model of the heterotetrameric ERMES complex. (B) Model 1: The lipid transporter Vps13 is recruited to artificial ER–mitochondria contacts. (C) Model 2: Mdm12 and Mdm34 are essential More about this image found in Models for ChiMERA rescue of ERMES depletion. (A) Model of the heterotetra...

Figure 2. Vps13 and joint loss of Mdm12-Mdm34 are not required for ERMES rescue by ChiMERA. (A) Schematic showing the model in which Vps13-Mcp1 can transfer lipids at ChiMERA-induced ER–mitochondria contact sites. (B) Representative images of More about this image found in Vps13 and joint loss of Mdm12-Mdm34 are not required for ERMES rescue by Ch...

Figure 3. Artificial tethering of Mmm1 to mitochondria can rescue loss of ERMES. (A) Schematic of model 3 showing that a Mmm1–Mdm10 dimer is functional upon additional tethering. (B) AlphaFold 3 prediction of Mmm1 and Mdm10 dimer. ChiMERA was More about this image found in Artificial tethering of Mmm1 to mitochondria can rescue loss of ERMES. (A) ...

Figure 4. SMP domain of Mmm1 is sufficient for rescue. (A) Spot assay of ERMES quadruple deletion yeast with and without the expression of artificially tethered ERMES members. (B) Representative images of mitochondria from the yeast in A. More about this image found in SMP domain of Mmm1 is sufficient for rescue. (A) Spot assay of ERMES quadr...

Figure 5. Rescue of ERMES loss by Mmm1’s SMP domain is independent of orientation and requires lipid transport activity. (A) Schematic of constructs used in B. (B) Spot assay of mmm1Δmdm12Δmdm34Δmdm10Δ yeast strains expressing tethering More about this image found in Rescue of ERMES loss by Mmm1’s SMP domain is independent of orientation and...

Figure 1. Changes in macrophage cell volume induce a large transcriptomic response that alters diverse cellular processes. (A and B) RNA-seq was performed on resting BMDMs from WT, VRAC KO, or CX3CR1 Cre–expressing controls (Cre), and WT and KO More about this image found in Changes in macrophage cell volume induce a large transcriptomic response th...

Figure 2. Changes in macrophage cell volume promote inflammation through type I Interferon signaling. (A–C) Heatmaps of mRNA expression of cytokines (A), interferons (B), and ISGs (C) from RNA-seq of WT or VRAC KO BMDMs ± incubation in More about this image found in Changes in macrophage cell volume promote inflammation through type I Inter...

Figure 3. Changes in cell volume drive IFNβ responses through a DNA- and TBK1-dependent pathway. (A and B) Western blot (A) and densitometry (B) of viperin in WT BMDMs incubated in iso-osmotic media (± the TLR3 agonist poly I:C [1 μg ml⁻¹]) or More about this image found in Changes in cell volume drive IFNβ responses through a DNA- and TBK1-depende...

Figure 4. Changes in cell volume induce translation arrest and stress granule formation, but type I IFN signaling is independent of RNA sensing through MAVS. (A) Puromycin incorporation assay in WT and VRAC KO BMDMs incubated in DMEM, More about this image found in Changes in cell volume induce translation arrest and stress granule formati...

Figure 5. Persistent cell swelling leads to caspase-3–dependent and type I IFN-independent cell death. (A and B) WT and VRAC KO BMDMs were incubated in iso-osmotic or hypo-osmotic media for the indicated time points. Cell viability was assessed More about this image found in Persistent cell swelling leads to caspase-3–dependent and type I IFN-indepe...

Figure 6. Cell volume disturbances and loss of VRAC potentiate STING signaling independent of cGAMP transport. (A) Schematic of the proposed role of VRAC in cGAMP transport. VRAC containing LRRC8A, LRRC8C, and LRRC8E can act as a conduit for More about this image found in Cell volume disturbances and loss of VRAC potentiate STING signaling indepe...

Figure 7. Cell volume regulates type I interferon production in response to diverse pathogen-mediated molecular patterns. (A–D) IFNβ release in the supernatant of WT and VRAC KO BMDMs incubated in DMEM or hypo-osmotic media (50% vol/vol H₂O in More about this image found in Cell volume regulates type I interferon production in response to diverse p...

Figure 8. Macrophage cell volume control influences antiviral responses to influenza A (IAV) infection. (A–C) WT and VRAC KO BMDMs were infected with IAV (MOI 10, 24 h) or mock-treated. qRT-PCR analysis for Ifnb (A), Cxcl10 (B), and Rsad2 (C) (n More about this image found in Macrophage cell volume control influences antiviral responses to influenza ...

Figure 9. Cell volume control mechanisms regulate inflammation in a murine model of hyperinflammation. (A) Schematic of the murine model of CpG-induced hyperinflammation. CpG-DNA (2 mg kg⁻¹), or PBS, was administered by i.p. injection on days 0, More about this image found in Cell volume control mechanisms regulate inflammation in a murine model of h...

Figure 1. Btsz is a Slp with multiple splice isoforms. (A) Cartoon depiction of a syncytial embryo during a nuclear cycle. Sagittal plane through a syncytial embryo (top row). Zoomed-in view of the actin caps in the dashed rectangular box where More about this image found in Btsz is a Slp with multiple splice isoforms. (A) Cartoon depiction of a sy...

Figure 2. Btsz is required for actin remodeling during syncytial blastoderm development. (A) Maximum projection of a top-down view of control and Btsz RNAi nuclear cycle 12 actin caps. Actin was visualized live using the mCh::MoeABD marker. (B) More about this image found in Btsz is required for actin remodeling during syncytial blastoderm developme...