1. Wide differences in the intensity of incident ultra violet energy are not accurately compensated by corresponding changes in the exposure time, so that the Bunsen-Roscoe reciprocity law does not hold, strictly, especially for bactericidal action on young, metabolically and genetically active bacteria. In the present series of experiments, however, the energies used at various wave lengths did not differ by so much as to cause a significant error in the reported reactions.
2. The longer wave length limit of a direct bactericidal action on S. aureus was found to be between 302 and 313 mµ. The shorter limit was not determined because the long exposures required vitiate quantitative results. Bactericidal action was observed at λ225 mµ.
3. The temperature coefficient of the bactericidal reaction approaches 1 and thus furnishes empirical evidence that the direct action of ultra violet light on bacteria is essentially physical or photochemical in character.
4. The hydrogen ion concentration of the environment has no appreciable effect upon the bactericidal reaction between the limits of pH 4.5 and 7.5. At pH 9 and 10 evidence of a slight but definite increase in bacterial susceptibility was noted, but this difference may have been due to a less favorable environment for subsequent recovery and multiplication of injured organisms.
5. Plane polarization of incident ultra violet radiation has no demonstrable effect upon its bactericidal action.
In a third paper of this group the ratios of incident to absorbed ultra violet energy at various wave lengths and the significance of these relations in an analysis of the bactericidal reaction will be discussed.