Page 302
Due to a typesetting error, the [ATP] column was deleted from Table. The corrected table appears below:
Hill Equation Parameters of X-InsP3R-1
| . | Ligand concentration . | [ATP] . | Pmax . | Kact . | Hact . | Kinh . | Hinh . |
|---|---|---|---|---|---|---|---|
| mM | nM | μM | |||||
| A | 100 nM AdA | 0.5 | 0.81 ± 0.03 | 200 ± 50 | 1.8 ± 0.3 | 45 ± 5 | 3.5 ± 0.4 |
| B | 10 μM InsP3 | 0.5 | 0.81 ± 0.03 | 190 ± 20 | 1.9 ± 0.3 | 54 ± 6 | 3.9 ± 0.7 |
| C | 0.5 nM AdA | 0.5 | 0.84 ± 0.03 | 250 ± 50 | 1.8 ± 0.3 | 8.9 ± 0.5 | 4.3 ± 0.7 |
| D | 33 nM InsP3 | 0.5 | 0.81 ± 0.03 | 190 ± 20 | 1.9 ± 0.3 | 11.0 ± 1.5 | 3.9 ± 0.7 |
| E | 20 nM InsP3 | 0.5 | 0.81 ± 0.03 | 190 ± 20 | 1.9 ± 0.3 | 0.21 ± 0.04 | 3.9 ± 0.7 |
| F | 10 μM InsP3 | 0 | 0.79 ± 0.02 | 420 ± 40 | 2.2 ± 0.3 | 110 ± 10 | 4.0 ± 0.7 |
| G | 33 nM InsP3 | 0 | 0.80 ± 0.05 | 540 ± 70 | 2.0 ± 0.3 | 1.4 ± 0.2 | 3.5 ± 0.7 |
| H | 100 nM AdA | 0 | 0.43 ± 0.03 | 400 ± 50 | 2.4 ± 0.3 | 130 ± 10 | 4.0 ± 0.7 |
| I | 20 nM AdA | 0 | 0.44 ± 0.03 | 440 ± 40 | 2.6 ± 0.6 | 9 ± 2 | 4.0 ± 0.7 |
| . | Ligand concentration . | [ATP] . | Pmax . | Kact . | Hact . | Kinh . | Hinh . |
|---|---|---|---|---|---|---|---|
| mM | nM | μM | |||||
| A | 100 nM AdA | 0.5 | 0.81 ± 0.03 | 200 ± 50 | 1.8 ± 0.3 | 45 ± 5 | 3.5 ± 0.4 |
| B | 10 μM InsP3 | 0.5 | 0.81 ± 0.03 | 190 ± 20 | 1.9 ± 0.3 | 54 ± 6 | 3.9 ± 0.7 |
| C | 0.5 nM AdA | 0.5 | 0.84 ± 0.03 | 250 ± 50 | 1.8 ± 0.3 | 8.9 ± 0.5 | 4.3 ± 0.7 |
| D | 33 nM InsP3 | 0.5 | 0.81 ± 0.03 | 190 ± 20 | 1.9 ± 0.3 | 11.0 ± 1.5 | 3.9 ± 0.7 |
| E | 20 nM InsP3 | 0.5 | 0.81 ± 0.03 | 190 ± 20 | 1.9 ± 0.3 | 0.21 ± 0.04 | 3.9 ± 0.7 |
| F | 10 μM InsP3 | 0 | 0.79 ± 0.02 | 420 ± 40 | 2.2 ± 0.3 | 110 ± 10 | 4.0 ± 0.7 |
| G | 33 nM InsP3 | 0 | 0.80 ± 0.05 | 540 ± 70 | 2.0 ± 0.3 | 1.4 ± 0.2 | 3.5 ± 0.7 |
| H | 100 nM AdA | 0 | 0.43 ± 0.03 | 400 ± 50 | 2.4 ± 0.3 | 130 ± 10 | 4.0 ± 0.7 |
| I | 20 nM AdA | 0 | 0.44 ± 0.03 | 440 ± 40 | 2.6 ± 0.6 | 9 ± 2 | 4.0 ± 0.7 |
Parameters for the biphasic Hill equations (Eq. 2) that fit the [Ca2+]i dependence of the Po of X-InsP3R-1 channel under various experimental conditions.
Page 304, right column, lines 1–6 of paragraph 2.
Due to an editorial error, parenthetical citation for Fig. 5 appears as A. The corrected sentence is given here:
The [Ca2+]i dependence of the AdA-liganded channel Po in the absence of ATP (Figs. 5 B and 6) was well fitted by the biphasic Hill equation (Eq. 2) with Kact, Hact, Kinh, and Hinh comparable with those for the channel activated by InsP3, but with a Pmax decreased to ∼0.4 (Table H).
Page 305, left column, lines 1–6 of paragraph 2.
Due to an editorial error, the row citation for Table was deleted. The corrected sentence is given here:
When the concentration of AdA was reduced from 100 to 20 nM in the absence of ATP, the channel became more sensitive to Ca2+ inhibition (Fig. 7), with only Kinh reduced while the other Hill equation parameters remained similar to those observed in 100 nM AdA (Table, row I).
