Page 302

Due to a typesetting error, the [ATP] column was deleted from Table. The corrected table appears below:

Table 1

Hill Equation Parameters of X-InsP3R-1

Ligand concentration[ATP]PmaxKactHactKinhHinh
  mM  nM  μM  
A 100 nM AdA 0.5 0.81 ± 0.03 200 ± 50 1.8 ± 0.3 45 ± 5 3.5 ± 0.4 
B 10 μM InsP3 0.5 0.81 ± 0.03 190 ± 20 1.9 ± 0.3 54 ± 6 3.9 ± 0.7 
C 0.5 nM AdA 0.5 0.84 ± 0.03 250 ± 50 1.8 ± 0.3 8.9 ± 0.5 4.3 ± 0.7 
D 33 nM InsP3 0.5 0.81 ± 0.03 190 ± 20 1.9 ± 0.3 11.0 ± 1.5 3.9 ± 0.7 
E 20 nM InsP3 0.5 0.81 ± 0.03 190 ± 20 1.9 ± 0.3 0.21 ± 0.04 3.9 ± 0.7 
F 10 μM InsP3 0 0.79 ± 0.02 420 ± 40 2.2 ± 0.3 110 ± 10 4.0 ± 0.7 
G 33 nM InsP3 0 0.80 ± 0.05 540 ± 70 2.0 ± 0.3 1.4 ± 0.2 3.5 ± 0.7 
H 100 nM AdA 0 0.43 ± 0.03 400 ± 50 2.4 ± 0.3 130 ± 10 4.0 ± 0.7 
I 20 nM AdA 0 0.44 ± 0.03 440 ± 40 2.6 ± 0.6 9 ± 2 4.0 ± 0.7 
Ligand concentration[ATP]PmaxKactHactKinhHinh
  mM  nM  μM  
A 100 nM AdA 0.5 0.81 ± 0.03 200 ± 50 1.8 ± 0.3 45 ± 5 3.5 ± 0.4 
B 10 μM InsP3 0.5 0.81 ± 0.03 190 ± 20 1.9 ± 0.3 54 ± 6 3.9 ± 0.7 
C 0.5 nM AdA 0.5 0.84 ± 0.03 250 ± 50 1.8 ± 0.3 8.9 ± 0.5 4.3 ± 0.7 
D 33 nM InsP3 0.5 0.81 ± 0.03 190 ± 20 1.9 ± 0.3 11.0 ± 1.5 3.9 ± 0.7 
E 20 nM InsP3 0.5 0.81 ± 0.03 190 ± 20 1.9 ± 0.3 0.21 ± 0.04 3.9 ± 0.7 
F 10 μM InsP3 0 0.79 ± 0.02 420 ± 40 2.2 ± 0.3 110 ± 10 4.0 ± 0.7 
G 33 nM InsP3 0 0.80 ± 0.05 540 ± 70 2.0 ± 0.3 1.4 ± 0.2 3.5 ± 0.7 
H 100 nM AdA 0 0.43 ± 0.03 400 ± 50 2.4 ± 0.3 130 ± 10 4.0 ± 0.7 
I 20 nM AdA 0 0.44 ± 0.03 440 ± 40 2.6 ± 0.6 9 ± 2 4.0 ± 0.7 

Parameters for the biphasic Hill equations (Eq. 2) that fit the [Ca2+]i dependence of the Po of X-InsP3R-1 channel under various experimental conditions.

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Page 304, right column, lines 1–6 of paragraph 2.

Due to an editorial error, parenthetical citation for Fig. 5 appears as A. The corrected sentence is given here:

The [Ca2+]i dependence of the AdA-liganded channel Po in the absence of ATP (Figs. 5 B and 6) was well fitted by the biphasic Hill equation (Eq. 2) with Kact, Hact, Kinh, and Hinh comparable with those for the channel activated by InsP3, but with a Pmax decreased to ∼0.4 (Table H).

Page 305, left column, lines 1–6 of paragraph 2.

Due to an editorial error, the row citation for Table was deleted. The corrected sentence is given here:

When the concentration of AdA was reduced from 100 to 20 nM in the absence of ATP, the channel became more sensitive to Ca2+ inhibition (Fig. 7), with only Kinh reduced while the other Hill equation parameters remained similar to those observed in 100 nM AdA (Table, row I).

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© 2001 The Rockefeller University Press
2001
The Rockefeller University Press