Hypertonic shrinkage of dog red cells caused rapid activation of Na/H exchange and rapid deactivation of [K-Cl] cotransport. Hypotonic swelling caused delayed deactivation of Na/H exchange and delayed activation of [K-Cl] cotransport. Okadaic acid stimulated shrinkage-induced Na/H exchange and inhibited swelling-induced [K-Cl] cotransport. The data are compatible with the kinetic model of Jennings and Al-Rohil (1990. J. Gen. Physiol. 95:1021-1040) for volume regulation of [K-Cl] cotransport in rabbit red cells and suggest that in dog red cells Na/H exchange and [K-Cl] cotransport are controlled by a common regulatory system. The proposal of Jennings and Schulz (1991. J. Gen. Physiol. 96:799-817) that activation/deactivation of volume-sensitive transport involves phosphorylation/dephosphorylation of a regulatory protein is supported by these observations.
Article|
November 01 1991
Coordinated regulation of shrinkage-induced Na/H exchange and swelling-induced [K-Cl] cotransport in dog red cells. Further evidence from activation kinetics and phosphatase inhibition.
J C Parker,
J C Parker
Department of Medicine, University of North Carolina, Chapel Hill 27599-7035.
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G C Colclasure,
G C Colclasure
Department of Medicine, University of North Carolina, Chapel Hill 27599-7035.
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T J McManus
T J McManus
Department of Medicine, University of North Carolina, Chapel Hill 27599-7035.
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J C Parker
Department of Medicine, University of North Carolina, Chapel Hill 27599-7035.
G C Colclasure
Department of Medicine, University of North Carolina, Chapel Hill 27599-7035.
T J McManus
Department of Medicine, University of North Carolina, Chapel Hill 27599-7035.
Online ISSN: 1540-7748
Print ISSN: 0022-1295
J Gen Physiol (1991) 98 (5): 869–880.
Citation
J C Parker, G C Colclasure, T J McManus; Coordinated regulation of shrinkage-induced Na/H exchange and swelling-induced [K-Cl] cotransport in dog red cells. Further evidence from activation kinetics and phosphatase inhibition.. J Gen Physiol 1 November 1991; 98 (5): 869–880. doi: https://doi.org/10.1085/jgp.98.5.869
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