Figure 1. B cells carrying low-affinity BCR are unable to seed GC in the NALT. (A) Schematic representation of the experimental setup shown in B–D. (B) AID-GFP mice were injected with Rosa26^tdTomato/+ B1-8^hi or B1-8^lo B cells mixed with More about this image found in B cells carrying low-affinity BCR are unable to seed GC in the NALT. (A) S...

Figure 2. Low-affinity B cell clones are unable to seed GCs in the NALT independent of competition. (A) MD4 mice were adoptively transferred with GFP⁺ B1-8^hi or Rosa26^tdTomato/+ B1-8^lo B cells. NALT and MedLN were removed and imaged by TPLSM on More about this image found in Low-affinity B cell clones are unable to seed GCs in the NALT independent o...

Figure 3. Formation of Tfh cells in the NALT depends on BCR affinity. (A–F) WT mice were injected with GFP⁺ B1-8^hi or Rosa26^tdTomato/+ B1-8^lo B cells mixed with CD45.1⁺ OT-II T cells. Flow cytometry analysis of OT-II T cells was performed at day More about this image found in Formation of Tfh cells in the NALT depends on BCR affinity. (A–F) WT mice ...

Figure 4. GC formation by high-affinity B cell clones depends on CCR6 expression. (A) WT mice were adoptively transferred with GFP⁺ B1-8^hi B cells and CD45.1⁺ OT-II T cells. CCR6 expression on B1-8^hi B cells was assessed by flow cytometry at the More about this image found in GC formation by high-affinity B cell clones depends on CCR6 expression. (A)...

Figure 5. IgA class switching in the NALT depends on CCR6-mediated B cell positioning in the SED. (A) CX₃CR1-GFP mice were adoptively transferred with Rosa26^tdTomato/+ B1-8^hi B cells and CD45.1⁺ OT-II T cells. NALTs were imaged on day 5 after More about this image found in IgA class switching in the NALT depends on CCR6-mediated B cell positioning...

Figure 6. CCR6 is required for bacteria-driven IgA GC B cell formation in the NALT. (A) Quantification by flow cytometry of B cell isotypes in NALTs from SPF or GF mice under homeostasis. IgA⁺, IgG2b⁺, and IgG1⁺ populations were gated from Fas⁺ More about this image found in CCR6 is required for bacteria-driven IgA GC B cell formation in the NALT. (...

Figure 1. LXR agonism exhibits treatment efficacy in breast and colon cancer models with low G-MDSC infiltration. (a) Abundance of Ly6G⁺ G-MDSCs across different syngeneic tumor models (n = 7, 7, 6, 8, and 6 for LLC, B16F10, E0771, E0771-DR, and More about this image found in LXR agonism exhibits treatment efficacy in breast and colon cancer models w...

Figure 2. LXR agonism promotes adaptive anti-tumor immunity in breast and colon cancer models. (a and b) Tumor growth and survival of naive mice or mice that had cleared E0771-DR tumors under LXR-agonism upon contralateral primary tumor (a) or More about this image found in LXR agonism promotes adaptive anti-tumor immunity in breast and colon cance...

Figure 3. LXR agonism enhances immune activation in tumor DLNs. (a–c) Phenotype of DLN-resident CD4⁺ and CD8⁺ T cells in E0771-cOC (a) or CT26-DR (c) tumor-bearing mice treated with RGX-104 (n = 8 and 7 per group, respectively, two-tailed t More about this image found in LXR agonism enhances immune activation in tumor DLNs. (a–c) Phenotype of D...

Figure 4. LXR agonistic therapy critically depends on CD11c ⁺ DCs and host Lxr but not Apoe expression. (a) Schematic of strategy for in vivo depletion of APC subsets. (b) Growth of orthotopic E0771-DR tumors in the presence or absence of More about this image found in LXR agonistic therapy critically depends on CD11c ⁺ DCs and ho...

Figure 5. LXR agonism promotes T cell activation. (a) Schematic of experimental approach to assess the impact of pharmacologic LXR activation on T cell priming and activation in vitro. (b) Gene ontology pathway enrichment analysis in genes More about this image found in LXR agonism promotes T cell activation. (a) Schematic of experimental appr...

Figure 6. LXR agonism renders CD8 ⁺ T cells more receptive to adopting effector functions through LXR activation in CD8 ⁺ T cells. (a) Expression of IFN-γ and granzyme B in OT-I CD8⁺ T cells after co-culture with antigen-pulsed BMDCs as More about this image found in LXR agonism renders CD8 ⁺ T cells more receptive to adopting e...

Figure 7. Transcriptional and chromatin accessibility profiling of naive CD8 ⁺ T cells upon LXR activation. (a) Volcano plot showing differentially expressed genes in naive CD8⁺ T cells treated with the LXR agonist RGX-104 for 24 h as assessed More about this image found in Transcriptional and chromatin accessibility profiling of naive CD8 ⁺ ...

Figure 8. LXR agonism and radiotherapy act additively. (a) Tumor growth of mice injected with E0771 tumor cells and treated with RGX-104 and either IgG control or anti-CD40 antibody (n = 7 per group). (b) Tumor volumes of mice from a on day 17 More about this image found in LXR agonism and radiotherapy act additively. (a) Tumor growth of mice inje...

Figure 9. RGX-104 treatment is associated with clonal T cell expansion and enhanced TCR diversity in relapsed/refractory cancer patients. (a) Fraction of patients receiving given therapies. (b) Number of expanded TCR clones in the peripheral More about this image found in RGX-104 treatment is associated with clonal T cell expansion and enhanced T...

Figure 1. Zbtb32 expression is selectively upregulated in T _tex tumor-infiltrating CD8 ⁺ T cells. (A) Fold change in the expression level of different TFs in T_pex and T_tex OT-I TILs in E.G7. (B) Volcanic plot showing the differential More about this image found in Zbtb32 expression is selectively upregulated in T ...

Figure 2. The CD28-PI3K axis induces Zbtb32 expression after CD8 ⁺ T cell activation. (A) The level of Zbtb32 expression was measured by RT-qPCR in activated CD8⁺ T cells in vitro (n = 3 for each group). (B) Quantifications of Zbtb32 More about this image found in The CD28-PI3K axis induces Zbtb32 expression after CD8 ...