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1-17 of 17
Irving L. Weissman
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Journal Articles
Yoon-Chi Han, Christopher Y. Park, Govind Bhagat, Jinping Zhang, Yulei Wang, Jian-Bing Fan, Mofang Liu, Yongrui Zou, Irving L. Weissman, Hua Gu
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2010) 207 (3): 475–489.
Published: 08 March 2010
Abstract
The function of microRNAs (miRNAs) in hematopoietic stem cells (HSCs), committed progenitors, and leukemia stem cells (LSCs) is poorly understood. We show that miR-29a is highly expressed in HSC and down-regulated in hematopoietic progenitors. Ectopic expression of miR-29a in mouse HSC/progenitors results in acquisition of self-renewal capacity by myeloid progenitors, biased myeloid differentiation, and the development of a myeloproliferative disorder that progresses to acute myeloid leukemia (AML). miR-29a promotes progenitor proliferation by expediting G1 to S/G2 cell cycle transitions. miR-29a is overexpressed in human AML and, like human LSC, miR-29a-expressing myeloid progenitors serially transplant AML. Our data indicate that miR-29a regulates early hematopoiesis and suggest that miR-29a initiates AML by converting myeloid progenitors into self-renewing LSC.
Includes: Supplementary data
Journal Articles
Deepta Bhattacharya, Agnieszka Czechowicz, A.G. Lisa Ooi, Derrick J. Rossi, David Bryder, Irving L. Weissman
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2009) 206 (12): 2837–2850.
Published: 02 November 2009
Abstract
Hematopoietic stem cells (HSCs) are thought to reside in discrete niches through stable adhesion, yet previous studies have suggested that host HSCs can be replaced by transplanted donor HSCs, even in the absence of cytoreductive conditioning. To explain this apparent paradox, we calculated, through cell surface phenotyping and transplantation of unfractionated blood, that ∼1–5% of the total pool of HSCs enters into the circulation each day. Bromodeoxyuridine (BrdU) feeding experiments demonstrated that HSCs in the peripheral blood incorporate BrdU at the same rate as do HSCs in the bone marrow, suggesting that egress from the bone marrow to the blood can occur without cell division and can leave behind vacant HSC niches. Consistent with this, repetitive daily transplantations of small numbers of HSCs administered as new niches became available over the course of 7 d led to significantly higher levels of engraftment than did large, single-bolus transplantations of the same total number of HSCs. These data provide insight as to how HSC replacement can occur despite the residence of endogenous HSCs in niches, and suggest therapeutic interventions that capitalize upon physiological HSC egress.
Includes: Multimedia, Supplementary data
Journal Articles
Marta Serafini, Scott J. Dylla, Masayuki Oki, Yves Heremans, Jakub Tolar, Yuehua Jiang, Shannon M. Buckley, Beatriz Pelacho, Terry C. Burns, Sarah Frommer, Derrick J. Rossi, David Bryder, Angela Panoskaltsis-Mortari, Matthew J. O'Shaughnessy, Molly Nelson-Holte, Gabriel C. Fine, Irving L. Weissman, Bruce R. Blazar, Catherine M. Verfaillie
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2007) 204 (7): 1729.
Published: 09 July 2007
Journal Articles
Marta Serafini, Scott J. Dylla, Masayuki Oki, Yves Heremans, Jakub Tolar, Yuehua Jiang, Shannon M. Buckley, Beatriz Pelacho, Terry C. Burns, Sarah Frommer, Derrick J. Rossi, David Bryder, Angela Panoskaltsis-Mortari, Matthew J. O'Shaughnessy, Molly Nelson-Holte, Gabriel C. Fine, Irving L. Weissman, Bruce R. Blazar, Catherine M. Verfaillie
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2007) 204 (1): 129–139.
Published: 16 January 2007
Abstract
For decades, in vitro expansion of transplantable hematopoietic stem cells (HSCs) has been an elusive goal. Here, we demonstrate that multipotent adult progenitor cells (MAPCs), isolated from green fluorescent protein (GFP)-transgenic mice and expanded in vitro for >40–80 population doublings, are capable of multilineage hematopoietic engraftment of immunodeficient mice. Among MAPC-derived GFP + CD45.2 + cells in the bone marrow of engrafted mice, HSCs were present that could radioprotect and reconstitute multilineage hematopoiesis in secondary and tertiary recipients, as well as myeloid and lymphoid hematopoietic progenitor subsets and functional GFP + MAPC-derived lymphocytes that were functional. Although hematopoietic contribution by MAPCs was comparable to control KTLS HSCs, approximately 10 3 -fold more MAPCs were required for efficient engraftment. Because GFP + host-derived CD45.1 + cells were not observed, fusion is not likely to account for the generation of HSCs by MAPCs.
Includes: Supplementary data
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2005) 203 (1): 73–85.
Published: 27 December 2005
Abstract
In the absence of irradiation or other cytoreductive conditioning, endogenous hematopoietic stem cells (HSCs) are thought to fill the unique niches within the bone marrow that allow maintenance of full hematopoietic potential and thus prevent productive engraftment of transplanted donor HSCs. By transplantation of purified exogenous HSCs into unconditioned congenic histocompatible strains of mice, we show that ∼0.1–1.0% of these HSC niches are available for engraftment at any given point and find no evidence that endogenous HSCs can be displaced from the niches they occupy. We demonstrate that productive engraftment of HSCs within these empty niches is inhibited by host CD4 + T cells that recognize very subtle minor histocompatibility differences. Strikingly, transplantation of purified HSCs into a panel of severe combined immunodeficient (SCID) mice leads to a rapid and complete rescue of lymphoid deficiencies through engraftment of these very rare niches and expansion of donor lymphoid progenitors. We further demonstrate that transient antibody-mediated depletion of CD4 + T cells allows short-term HSC engraftment and regeneration of B cells in a mouse model of B(-) non-SCID. These experiments provide a general mechanism by which transplanted HSCs can correct hematopoietic deficiencies without any host conditioning or with only highly specific and transient lymphoablation.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2005) 202 (11): 1599–1611.
Published: 05 December 2005
Abstract
Knowledge of the molecular networks controlling the proliferation and fate of hematopoietic stem cells (HSC) is essential to understand their function in maintaining blood cell production during normal hematopoiesis and upon clinical transplantation. Using highly purified stem and progenitor cell populations, we define the proliferation index and status of the cell cycle machinery at discrete stages of hematopoietic differentiation and during cytokine-mediated HSC mobilization. We identify distinct sets of cell cycle proteins that specifically associate with differentiation, self-renewal, and maintenance of quiescence in HSC and progenitor cells. Moreover, we describe a striking inequality of function among in vivo cycling and quiescent HSC by demonstrating that their long-term engraftment potential resides predominantly in the G 0 fraction. These data provide a direct link between HSC proliferation and function and identify discrete molecular targets in regulating HSC cell fate decisions that could have implications for both the therapeutic use of HSC and the understanding of leukemic transformation.
Includes: Supplementary data
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2005) 201 (10): 1579–1589.
Published: 16 May 2005
Abstract
Several studies have reported that bone marrow (BM) cells may give rise to neurons and astrocytes in vitro and in vivo. To further test this hypothesis, we analyzed for incorporation of neural cell types expressing donor markers in normal or injured brains of irradiated mice reconstituted with whole BM or single, purified c-kit + Thy1.1 lo Lin − Sca-1 + (KTLS) hematopoietic stem cells (HSCs), and of unirradiated parabionts with surgically anastomosed vasculature. Each model showed low-level parenchymal engraftment of donor-marker + cells with 96–100% immunoreactivity for panhematopoietic (CD45) or microglial (Iba1 or Mac1) lineage markers in all cases studied. Other than one arborizing structure in the olfactory bulb of one BM-transplanted animal, possibly representing a neuronal or glial cell process, we found no donor-marker–expressing astrocytes or non-Purkinje neurons among >10,000 donor-marker + cells from 21 animals. These data strongly suggest that HSCs and their progeny maintain lineage fidelity in the brain and do not adopt neural cell fates with any measurable frequency.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2003) 198 (2): 305–313.
Published: 21 July 2003
Abstract
Stimulation of Flt3 receptor tyrosine kinase through its cognate ligand expands early hematopoietic progenitor and dendritic cells (DCs) in humans and mice. The exact developmental stages at which hematopoietic progenitors express Flt3, are responsive to its ligand, and subsequently develop to DCs, are not known. Here we show that common lymphoid and common myeloid progenitors, as well as steady state DCs in thymus, spleen, and epidermis, express Flt3. The receptor is down-regulated once definitive B cell, T cell, and megakaryocyte/erythrocyte commitment occurs, and Flt3 is not detectable on other steady state hematopoietic cell populations. Upon in vivo Flt3 ligand (Flt3L) administration, Flt3 + progenitor cells and their progeny DCs are expanded, whereas Flt3 − downstream progenitors are not, or are only slightly increased. Transplantation of common lymphoid and common myeloid progenitors and subsequent Flt3L injection increases progeny DCs of both precursor populations. These findings provide a definitive map of Flt3 expression in the hematopoietic hierarchy and directly demonstrate that Flt3L can drive DC development along both the lymphoid and myeloid developmental pathways from Flt3 + progenitors to Flt3 + DCs.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2002) 196 (11): 1427–1433.
Published: 02 December 2002
Abstract
Telomeres shorten in hematopoietic cells, including hematopoietic stem cells (HSCs), during aging and after transplantation, despite the presence of readily detectable levels of telomerase in these cells. In T cells, antigenic stimulation has been shown to result in a marked increase in the level of telomerase activity. We now show that stimulation of T cells derived from serially transplanted HSC results in a telomerase-dependent elongation of telomere length to a size similar to that observed in T cells isolated directly from young mice. Southern analysis of telomere length in resting and anti-CD3/CD28 stimulated donor-derived splenic T cells revealed an increase in telomere size by ∼7 kb for the population as a whole. Stimulation of donor-derived T cells from recipients of HSCs from telomerase-deficient mice did not result in regeneration of telomere length, demonstrating a dependence on telomerase. Furthermore, clonal anti-CD3/CD28 stimulation of donor-derived T cells followed by fluorescent in situ hybridization (FISH) analysis of telomeric signal intensity showed that telomeres had increased in size by ∼50% for all clonal expansions. Together, these results imply that one role for telomerase in T cells may be to renew or extend replicative potential via the rejuvenation of telomere length.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2002) 195 (9): 1145–1154.
Published: 06 May 2002
Abstract
Although hematopoietic stem cell (HSC) migration into and out of sites of active hematopoiesis is poorly understood, it is a critical process that underlies modern clinical stem cell transplantation and may be important for normal hematopoietic homeostasis. Given the established roles of chemotactic cytokine (chemokine)-directed migration of other leukocyte subsets, the migration of murine HSC to a large panel of CC and CXC chemokines was investigated. HSC migrated only in response to stromal derived factor-1α, the ligand for the CXC chemokine receptor 4 (CXCR4). CXCR4 expression by HSC was confirmed by reverse transcription polymerase chain reaction analysis. Surprisingly, HSC also expressed mRNA for CCR3 and CCR9, although they failed to migrate to the ligands for these receptors. The sharply restricted chemotactic responsiveness of HSC is unique among leukocytes and may be necessary for the specific homing of circulating HSC to bone marrow, as well as for the maintenance of HSC in hematopoietic microenvironments.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2001) 193 (8): 917–924.
Published: 09 April 2001
Abstract
Reactivation of telomerase and maintenance of telomere length can lead to the prevention of replicative senescence in some human somatic cells grown in vitro. To investigate whether telomere shortening might also play a role in the limitation of hematopoietic stem cell (HSC) division capacity in vivo, we analyzed telomere length during serial transplantation of murine HSCs. Southern blot analysis of telomere length in donor bone marrow cells revealed extensive shortening (∼7 kb) after just two rounds of HSC transplantation. The number of cycling HSCs increased after transplantation and remained elevated for at least 4 mo, while the frequency of HSCs in the bone marrow was completely regenerated by 2 mo after transplantation. Direct analysis of telomeres in HSCs by fluorescent in situ hybridization during serial transplantation also revealed a reduction in telomere size. Together, these data show that telomeres shorten during division of HSCs in vivo , and are consistent with the hypothesis that telomere shortening may limit the replicative capacity of HSCs.
Journal Articles
Scott C. Kogan, Diane E. Brown, David B. Shultz, Bao-Tran H. Truong, Valerie Lallemand-Breitenbach, Marie-Claude Guillemin, Eric Lagasse, Irving L. Weissman, J. Michael Bishop
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2001) 193 (4): 531–544.
Published: 20 February 2001
Abstract
The promyelocytic leukemia retinoic acid receptor α (PMLRARα) chimeric protein is associated with acute promyelocytic leukemia (APL). PMLRARα transgenic mice develop leukemia only after several months, suggesting that PMLRARα does not by itself confer a fully malignant phenotype. Suppression of apoptosis can have a central role in tumorigenesis; therefore, we assessed whether BCL-2 influenced the ability of PMLRARα to initiate leukemia. Evaluation of preleukemic animals showed that whereas PMLRARα alone modestly altered neutrophil maturation, the combination of PMLRARα and BCL-2 caused a marked accumulation of immature myeloid cells in bone marrow. Leukemias developed more rapidly in mice coexpressing PMLRARα and BCL-2 than in mice expressing PMLRARα alone, and all mice expressing both transgenes succumbed to leukemia by 7 mo. Although both preleukemic, doubly transgenic mice and leukemic animals had abundant promyelocytes in the bone marrow, only leukemic mice exhibited thrombocytopenia and dissemination of immature cells. Recurrent gain of chromosomes 7, 8, 10, and 15 and recurrent loss of chromosome 2 were identified in the leukemias. These chromosomal changes may be responsible for the suppression of normal hematopoiesis and dissemination characteristic of the acute leukemias. Our results indicate that genetic changes that inhibit apoptosis can cooperate with PMLRARα to initiate APL.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2000) 192 (12): 1707–1718.
Published: 11 December 2000
Abstract
Growth factors can cause cells to proliferate, differentiate, survive, or die. Distinguishing between these responses is difficult in multicellular, multiparameter systems. Yet this is essential to understand the impact on cells like hematopoietic stem cells (HSCs), which have strict and still poorly understood growth factor requirements. Single cell plating in serum-free medium allows direct assessment of growth factor responses. The range of tested factors can be expanded if the cells are protected from growth factor deprivation–induced apoptosis. BCL-2 is overexpressed in HSCs of H2K- BCL -2 transgenic mice, protecting them from many apoptotic stimuli. The response of single wild-type and transgenic HSCs to stimulations with individual factors was tested. Surprisingly, we find that high level BCL-2 expression does not prevent rapid death under serum-free conditions, even though it does in the presence of serum. We also find that transgenic, but not wild-type cells, survive and proliferate rapidly in response to steel factor (Kit ligand). These studies show that two separate signals are necessary to prevent apoptosis in HSCs, and that Kit ligand by itself provides a strong proliferative stimulus to HSCs. However, the proliferative response does not result in self-renewal, but in differentiation to all known hematopoietic oligolineage progenitors.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2000) 191 (2): 253–264.
Published: 17 January 2000
Abstract
Hematopoietic stem cells (HSC) give rise to cells of all hematopoietic lineages, many of which are short lived. HSC face developmental choices: self-renewal (remain an HSC with long-term multilineage repopulating potential) or differentiation (become an HSC with short-term multilineage repopulating potential and, eventually, a mature cell). There is a large overcapacity of differentiating hematopoietic cells and apoptosis plays a role in regulating their numbers. It is not clear whether apoptosis plays a direct role in regulating HSC numbers. To address this, we have employed a transgenic mouse model that overexpresses BCL-2 in all hematopoietic cells, including HSC: H2K- BCL -2. Cells from H2K- BCL -2 mice have been shown to be protected against a wide variety of apoptosis-inducing challenges. This block in apoptosis affects their HSC compartment. H2K- BCL -2–transgenic mice have increased numbers of HSC in bone marrow (2.4× wild type), but fewer of these cells are in the S/G 2 /M phases of the cell cycle (0.6× wild type). Their HSC have an increased plating efficiency in vitro, engraft at least as well as wild-type HSC in vivo, and have an advantage following competitive reconstitution with wild-type HSC.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (1998) 187 (11): 1813–1823.
Published: 01 June 1998
Abstract
In the principal pathway of α/β T cell maturation, T cell precursors from the bone marrow migrate to the thymus and proceed through several well-characterized developmental stages into mature CD4 + and CD8 + T cells. This study demonstrates an alternative pathway in which the bone marrow microenvironment also supports the differentiation of T cell precursors into CD4 + and CD8 + T cells. The marrow pathway recapitulates developmental stages of thymic maturation including a CD4 + CD8 + intermediary cell and positive and negative selection, and is strongly inhibited by the presence of mature T cells. The contribution of the marrow pathway in vivo requires further study in mice with normal and deficient thymic or immune function.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (1973) 137 (2): 504–510.
Published: 01 February 1973
Abstract
Outer thymic cortical large lymphocytes were labeled by transcapsular administration of tritiated thymidine. By 2–4 days after labeling, small and medium labeled lymphocyte descendants were found throughout the cortex and in the medulla. The labeled cortical lymphocytes undergo pycnosis after parenteral administration of hydrocortisone 24 h previously, but their (labeled) medullary descendants do not. In addition, parenteral administration of hydrocortisone at the time of surface labeling results in the absence of the appearance of labeled medullary descendants.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (1967) 126 (2): 291–304.
Published: 01 August 1967
Abstract
The preceding studies have established the following points: Intrathymic labeling of thymic lymphocytes provides an adequate marker system to detect the migration of thymus cells to peripheral lymphoid sites. In the newborn, this comprises a major portion of the total lymphocyte population in lymph nodes and spleen. In the adult, this migration is limited to specific portions of lymph nodes and spleen, i.e., those portions which serve the recirculating pool of small lymphocytes. Kinetic studies of labeling within the adult thymus indicate that large cells give rise to medium and small cells, which then migrate to the specific sites noted above. In the newborn, the kinetic pattern is similar to that of adults, with the single distinction that large cells also migrate, accelerating the tempo of migration in these hosts. The long-term fate and function of thymus cell migrants has not yet been determined.