Figure 1. GSK126 suppresses MyD88-dependent proinflammatory responses in macrophages/microglia . (A–D) Flow cytometry of the surface CD11b and F4/80 expression and MTT analysis of primary cultured bone marrow–derived macrophages (A and B) or More about this image found in GSK126 suppresses MyD88-dependent proinflammatory responses in macrophages/...

Figure 2. Ezh2 deficiency neither affects the development and maturation of myeloid cells nor influences the activation of peripheral lymphoid cells. (A) Genotyping PCR analysis of tail DNA from Ezh2^f/f, Ezh2^+/+, Ezh2^f/+, and LysM-cre mice. (B) More about this image found in Ezh2 deficiency neither affects the development and matura...

Figure 3. Ezh2 deficiency in myeloid cells suppresses DSS-induced colitis . (A) qRT-PCR analysis of Ezh2 mRNA in FACS-sorted CD11b⁺F4/80⁺macrophages from the colon and spleen of naive WT and Ezh2^M−/− mice. (B) The body-weight loss of WT and More about this image found in Ezh2 deficiency in myeloid cells suppresses DSS-induced co...

Figure 4. Microglia Ezh2 facilitates CNS autoimmune inflammation. (A) Mean clinical scores of age- and sex-matched WT and myeloid cell Ezh2-deficient (Ezh2^M−/−) mice after the induction of EAE with MOG_35–55 (n = 5 mice per group). (B) H&E More about this image found in Microglia Ezh2 facilitates CNS autoimmune inflammation. (A) Mean clinical ...

Figure 5. Ezh2 mediates MyD88-dependent inflammatory responses in macrophages/microglia. (A) GO term analysis of Ezh2 function in macrophages treated with LPS through DAVID informatics shows that the most significantly enriched biological More about this image found in Ezh2 mediates MyD88-dependent inflammatory responses in macrophages/microgl...

Figure 6. Ezh2 epigenetically controls Socs3 expression in macrophages/microglia. (A) qRT-PCR analysis of the indicated genes in WT and Ezh2-deficient macrophages that were pretreated with DMSO or cycloheximide (CHX) for 1 h and then left More about this image found in Ezh2 epigenetically controls Socs3 expression in macrophages/microglia. (A)...

Figure 7. Ezh2 regulates TRAF6 degradation and its downstream signaling. (A and B) Analysis of TRAF6 Lys48-linked ubiquitination in WT and Ezh2-deficient macrophages (A) or in Ezh2-KO BV2 cell that infected with EV or lentiviral vector encoding More about this image found in Ezh2 regulates TRAF6 degradation and its downstream signaling. (A and B) A...

Figure 8. Inhibition of Socs3 restores autoimmune inflammation in Ezh2-deficient mice. (A) qRT-PCR determining the relative expression of the indicated genes in WT and Ezh2-deficient macrophages that infected with retrovirus carrying control More about this image found in Inhibition of Socs3 restores autoimmune inflammation in Ezh2...

Figure 9. Model of Ezh2 in the regulation of macrophages/microglia activation and autoimmune inflammation. In WT macrophages/microglia, Ezh2, together with EED and Suz12 form PRC2 complex, which directly target Socs3 to promote the H3K27me3 and More about this image found in Model of Ezh2 in the regulation of macrophages/microglia activation and aut...

Figure 1. Mtb infection induces accumulation of interstitial macrophages. To characterize lung macrophage populations and determine the relative distribution of Mtb within the phagocyte subsets of the murine lung, tissue was harvested from WT More about this image found in Mtb infection induces accumulation of interstitial macrophages. To charact...

Figure 2. Recruited monocytes give rise to lung interstitial macrophages in Mtb infection. Accumulation of IMs in infected lung tissue is impaired in CCL2^−/− mice and in mice that have been treated with clodronate liposomes to deplete their More about this image found in Recruited monocytes give rise to lung interstitial macrophages in Mtb infec...

Figure 3. Mtb induces local proliferation of lung macrophages. Lung cells isolated at indicated time points were probed with an antibody against the nuclear replication marker Ki67. Both uninfected bystander AMs and IMs exhibited an increased More about this image found in Mtb induces local proliferation of lung macrophages. Lung cells isolated a...

Figure 4. Reporter Mtb strains reveal distinct fitness states of Mtb in lung phagocytes. The fitness and replication status of Mtb in the different host phagocyte subsets was determined using fluorescent Mtb reporter strains and Mtb carrying a More about this image found in Reporter Mtb strains reveal distinct fitness states of Mtb in lung phagocyt...

Figure 5. Selective depletion of AMs and IMs results in a decrease or an increase in bacterial burden, respectively. Treatment of mice by clodronate liposomes delivered either i.n. or i.v. to deplete the AMs or circulating monocytes, More about this image found in Selective depletion of AMs and IMs results in a decrease or an increase in ...

Figure 6. Transcriptional profiles of AMs and IMs during Mtb infection. RNA-sequencing analysis revealed distinct transcriptional profiles of AMs and IMs sorted from mice infected with mCherry-Mtb for 2 wk. AM+TB and IM+TB, cells with Mtb. AM-TB More about this image found in Transcriptional profiles of AMs and IMs during Mtb infection. RNA-sequenci...

Figure 7. Metabolic states of lung macrophage subsets in Mtb infection. IMs are in a glycolytically active state, whereas AMs exhibit increased fatty acid uptake and β-oxidation of long chain fatty acids. Depletion of IMs through treatment with More about this image found in Metabolic states of lung macrophage subsets in Mtb infection. IMs are in a...

Figure 8. Different metabolic pathways in macrophages regulate Mtb growth differentially in vitro. Inhibition of glycolysis by 2-DG promotes Mtb growth in BMDMs, whereas inhibition of fatty acid oxidation by ETO suppresses Mtb growth. (A) CFU More about this image found in Different metabolic pathways in macrophages regulate Mtb growth differentia...

Figure 9. Models of macrophage reprogramming and preprogramming. (A and B) Schematic representation illustrating how macrophage function in the reprogramming model (A) is determined by immune signaling within the tissue niche. In the proposed More about this image found in Models of macrophage reprogramming and preprogramming. (A and B) Schematic...