The virus of Newcastle disease of chickens resembles those of the encephalitis group in its ability to spread throughout the developing egg and embryo, but it is similar to influenza virus in the high concentration of it found in the allantoic fluid before death. No effect of the size of the inoculum on the final titer of virus in the allantoic fluid was detected. Good growth occurred at temperatures from 35° to 41°C., apparently more rapid at 40°C. than at 35°C. No appreciable development of virus capable of agglutinating red cells but of low embryo infectivity was found. Although virus multiplication was not immediately perceptible after inoculation, this cannot on present evidence be attributed to a real lag phase.

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