THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT

Pillemer , L.; Ecker , E. E.; Oncley , J. L.; Cohn , E. J.

doi:10.1084/jem.74.4.297

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Article| October 01 1941

THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT

L. Pillemer,

L. Pillemer

From the Department of Physical Chemistry, Harvard Medical School, Boston, and the Institute of Pathology, Western Reserve University, and the University Hospitals, Cleveland

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E. E. Ecker,

E. E. Ecker

From the Department of Physical Chemistry, Harvard Medical School, Boston, and the Institute of Pathology, Western Reserve University, and the University Hospitals, Cleveland

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J. L. Oncley,

J. L. Oncley

From the Department of Physical Chemistry, Harvard Medical School, Boston, and the Institute of Pathology, Western Reserve University, and the University Hospitals, Cleveland

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E. J. Cohn

From the Department of Physical Chemistry, Harvard Medical School, Boston, and the Institute of Pathology, Western Reserve University, and the University Hospitals, Cleveland

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Author and Article Information

L. Pillemer

From the Department of Physical Chemistry, Harvard Medical School, Boston, and the Institute of Pathology, Western Reserve University, and the University Hospitals, Cleveland

E. E. Ecker

From the Department of Physical Chemistry, Harvard Medical School, Boston, and the Institute of Pathology, Western Reserve University, and the University Hospitals, Cleveland

J. L. Oncley

From the Department of Physical Chemistry, Harvard Medical School, Boston, and the Institute of Pathology, Western Reserve University, and the University Hospitals, Cleveland

E. J. Cohn

From the Department of Physical Chemistry, Harvard Medical School, Boston, and the Institute of Pathology, Western Reserve University, and the University Hospitals, Cleveland

Received: June 04 1941

Online ISSN: 1540-9538

Print ISSN: 0022-1007

Copyright, 1941, by The Rockefeller Institute for Medical Research New York

1941

J Exp Med (1941) 74 (4): 297–308.

https://doi.org/10.1084/jem.74.4.297

1. Methods for the separation from guinea pig serum in highly purified form of three of the components of complement are described. These substances are the so called mid-piece, end-piece, and 4th component.

2. Mid-piece has been separated as a euglobulin, with an electrophoretic mobility of 2.9 x 10^–5 in phosphate buffer of ionic strength 0.2 at pH 7.7, and with a sedimentation constant of 6.4 x 10^–13 in potassium chloride of ionic strength 0.2.

3. End-piece and 4th component were found together in a euglobulin fraction of serum which contained 10.3 per cent carbohydrate and had an electrophoretic mobility of 4.2 x 10^–5 in phosphate buffer of ionic strength 0.2 at pH 7.7.

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Copyright, 1941, by The Rockefeller Institute for Medical Research New York

1941

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