The effect of heat, hypo- and hypertonic salt solutions, glycerin, desiccation, and mechanical injury was studied on leukemic lymphoid cells of the mouse, and the leukemia-transmitting property of cell suspensions (hypothetical transmitting agent) in vitro.

The results indicate that leukemia has not been transmitted by inoculation without the introduction of living cells. The interval between inoculation and death bears an inverse relationship to the number of living cells inoculated.

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