STUDIES ON VITAL STAINING I. SOME PROBLEMS IN COLORIMETRY. THE QUANTITATIVE ANALYSIS OF MIXTURES OF COLORED SUBSTANCES IN SOLUTION

Smith , H. P.

doi:10.1084/jem.51.3.369

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Article| March 01 1930

STUDIES ON VITAL STAINING : I. SOME PROBLEMS IN COLORIMETRY. THE QUANTITATIVE ANALYSIS OF MIXTURES OF COLORED SUBSTANCES IN SOLUTION

H. P. Smith

From the Department of Pathology, School of Medicine and Dentistry, University of Rochester, Rochester, N. Y.

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Author and Article Information

H. P. Smith

From the Department of Pathology, School of Medicine and Dentistry, University of Rochester, Rochester, N. Y.

Received: November 15 1929

Online ISSN: 1540-9538

Print ISSN: 0022-1007

Copyright, 1930, by The Rockefeller Institute for Medical Research New York

1930

J Exp Med (1930) 51 (3): 369–377.

https://doi.org/10.1084/jem.51.3.369

1. A spectrophotometric method is discussed which permits quantitative analysis of colored substances present in mixtures.

2. Special attention is given to the analysis of mixtures of two dyes which are being used in a series of studies on vital staining.

3. It is shown that the method can be applied to quantitative analysis of mixtures of naturally occurring animal pigments or to mixtures of these with various other colored substances.

4. Certain limitations of the method and certain necessary precautions are discussed.

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Copyright, 1930, by The Rockefeller Institute for Medical Research New York

1930

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STUDIES ON VITAL STAINING : I. SOME PROBLEMS IN COLORIMETRY. THE QUANTITATIVE ANALYSIS OF MIXTURES OF COLORED SUBSTANCES IN SOLUTION

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