From the foregoing evidence, specific bacterial agglutination may be conceived of as follows: When bacteria are mixed with their homologous agglutinative sera, specific union between organism and agglutinin occurs. This interaction consists of specific coating of the bacteria by globulin. By virtue of the fact, noted by Loeb and illustrated in Fig. 6, that protein film formation gives the coated particle the characteristics of denatured protein, the bacteria now take on the character of particles of denatured globulin. Particles of denatured protein flocculate whenever their charge is reduced by electrolyte to a critical level lying somewhere between 12 and 14 millivolts. This is true even when the salt is strong, as, unlike bacterial particles, their cohesive force is not readily depressed by salt. The sensitized bacteria, now being essentially particles of denatured protein, likewise agglutinate as soon as their charge is reduced by electrolyte to this potential level.
Article| November 01 1926
STUDIES IN AGGLUTINATION : III. ON THE MECHANISM OF THE AGGLUTINATION OF BACTERIA BY SPECIFIC AGGLUTINATING SERUM.
Gerald S. Shibley
From the Department of Medicine, College of Physicians and Surgeons of Columbia University and the Presbyterian Hospital, New York.
Received: June 22 1926
Online Issn: 1540-9538
Print Issn: 0022-1007
Copyright, 1926, by The Rockefeller Institute for Medical Research New York
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Gerald S. Shibley; STUDIES IN AGGLUTINATION : III. ON THE MECHANISM OF THE AGGLUTINATION OF BACTERIA BY SPECIFIC AGGLUTINATING SERUM. . J Exp Med 1 November 1926; 44 (5): 667–681. doi: https://doi.org/10.1084/jem.44.5.667
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