1. Calcium chloride given subcutaneously, intraperitoneally, or intravenously has been found to have no effect upon the production of botulism following the injection of Bacillus botulinus (Strain 80B) into the peritoneal cavity of guinea pigs.
2. Treatment of Bacillus botulinus with alcohol has been found markedly to decrease its toxicity for guinea pigs. This is in conformity with the work of Bronfenbrenner and Schlesinger.
3. Toxin-free spores of Bacillus botulinus have been found pathogenic for guinea pigs.
4. No prejudice as to possible results in rabbits should be based upon the above conclusions.