A simple and convenient method is described for the preparation and use of collodion sacs for aerobic and anaerobic bacterial cultivation in vitro.

The sacs are suitable for the study of various problems in bacterial growth and metabolism. By their use with the Smith-Noguchi tissue medium, certain microorganisms may be grown in the absence of the confusing, antigenic protein precipitate characteristic of cultures containing fresh tissue, and thus the organisms are obtained in a suitable condition for serological study.

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