It seems clearly established that non-hemolytic streptococci are responsible for a considerable number of cases of bovine mastitis. Of the 81 animals examined, 31 were suffering from infections of this type. The lesions produced in invaded quarters varied from an involvement of only the lining epithelium of the large milk ducts to severe degeneration and necrosis of the secreting epithelium. In one instance a considerable portion of the glandular elements had been replaced with connective tissue.
The streptococci fall into two groups when their action on the various carbohydrates is considered. Thirty-four strains fermented dextrose, lactose, saccharose, maltose, and salicin; five others attacked the first four sugars but failed to produce acid in salicin. All mastitis streptococci failed to act upon raffinose, inulin, or mannite. One species isolated from a mammary abscess produced acid in all the carbohydrates.
All the strains were agglutinated with an antiserum prepared from one typical strain. The agglutination titer varied over wide limits, although all the streptococci were agglutinated at a dilution of 1:500. None of the strains inoculated proved pathogenic for rabbits. A pig fed on the milk from two typical cases of mastitis remained well.