1. Intestinal secretion, collected by the method described in this paper, is non-toxic when fresh.
2. This secretion, when heated immediately to 90–95°C. and kept sterile, remains non-toxic.
3. This secretion, when not heated, remains non-toxic when kept under chloroform and toluene, even if incubated at 37°C.
4. This secretion, when not heated, but collected in a sterile flask, becomes toxic upon incubating 18 hours, producing symptoms like those of closed loop fluid.
5. The secretion, when treated with chloroform and toluene, and later incubated for 18 hours, after these preservatives have been removed by distillation at 60°C., does not produce lesions typical of closed loop fluid.