Correction: Cytosolic PCNA interacts with p47phox and controls NADPH oxidase NOX2 activation in neutrophils

Absence of T2AA antiinflammatory effect in TNBS-induced colitis following neutrophil depletion. (A) Experimental protocol of colitis after neutrophil depletion using anti-Ly6G mAb. (B and C) Flow cytometry quantification of neutrophils in blood (left panel) and in bone marrow (right panel) in control (IgG) and neutrophil-depleted (Ly6G) mice. (C) Macroscopic changes following TNBS treatment measured by Wallace score. The data are mean ± SEM (ANOVA). (D) Representative photomicrographs of neutrophil immunostaining of colonic tissues with the neutrophil marker recovered from control (IgG) and neutrophil-depleted mice (Ly6G) treated with TNBS alone or TNBS combined with T2AA. The scale bars correspond to 20 µm, magnification 200×. (E) Quantification of neutrophil immunostaining as described in Materials and methods. In B, C, and E, the data are mean ± SEM. Two independent sets of experiments were performed with a total of eight mice per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001, ANOVA.