Cytolytic CD8⁺ T Cells Recognizing CFP10 Are Recruited to the Lung after Mycobacterium tuberculosis Infection

Optimum immunity against Mycobacterium tuberculosis requires both CD4⁺ and CD8⁺ T cells. In contrast with CD4⁺ T cells, few antigens are known that elicit CD8⁺ T cells during infection. CD8⁺ T cells specific for culture filtrate protein-10 (CFP10) are found in purified protein derivative positive donors, suggesting that CFP10 primes CD8⁺ T cells in vivo. Using T cells from M. tuberculosis–infected mice, we identified CFP10 epitopes recognized by CD8⁺ T cells and CD4⁺ T cells. CFP10-specific T cells were detected as early as week 3 after infection and at their peak accounted for up to 30% of CD8⁺ T cells in the lung. IFNγ-producing CD8⁺ and CD4⁺ T cells recognizing CFP10 epitopes were preferentially recruited to the lungs of M. tuberculosis–infected mice. In vivo cytolytic activity of CD8⁺ T cells specific for CFP10 and TB10.3/10.4 proteins was detected in the spleen, pulmonary lymph nodes, and lungs of infected mice. The cytolytic activity persisted long term and could be detected 260 d after infection. This paper highlights the cytolytic function of antigen-specific CD8⁺ T cells elicited by M. tuberculosis infection and demonstrates that large numbers of CFP10-specific cytolytic CD8⁺ T cells are recruited to the lung after M. tuberculosis infection.