Since some murine cells expressing human CD4 fail to internalize HIV-1, another block was thought to be located at the level of viral entry in addition to CD4. Recently, CXCR4 was shown to function as a coreceptor for T cell line-tropic HIV-1 entry. Here we demonstrated that cells expressing murine CXCR4 and human CD4 fused with cells expressing the env proteins derived from T cell line-tropic HIV-1 and were infected with T cell line-tropic HIV-1 strains. In contrast, the same cells were not infected with chimeric clones constructed by substitution of monocyte- or macrophage-tropic strain-derived env region or V3 region into T cell line-tropic HIV-1, indicating V3 loop of envelope protein is required for murine CXCR4mediated HIV-1 entry. We conclude that murine CXCR4 is not a species specific barrier to the entry of T cell line-tropic HIV-1.
Skip Nav Destination
Article navigation
19 May 1997
Article Contents
Brief Definitive Report|
May 19 1997
CXCR4/fusin Is Not a Species-specific Barrier in Murine Cells for HIV-1 Entry
Kazunobu Tachibana,
Kazunobu Tachibana
From the *Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 590-02, Japan; ‡Shionogi Institute for Medical Science, Osaka 566, Japan; §Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan; ‖Department of Molecular Preventive Medicine, Division of Social Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and ¶Department of Medicine III, Osaka University Medical School, Osaka 565, Japan
Search for other works by this author on:
Toshihiro Nakajima,
Toshihiro Nakajima
From the *Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 590-02, Japan; ‡Shionogi Institute for Medical Science, Osaka 566, Japan; §Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan; ‖Department of Molecular Preventive Medicine, Division of Social Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and ¶Department of Medicine III, Osaka University Medical School, Osaka 565, Japan
Search for other works by this author on:
Akihiko Sato,
Akihiko Sato
From the *Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 590-02, Japan; ‡Shionogi Institute for Medical Science, Osaka 566, Japan; §Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan; ‖Department of Molecular Preventive Medicine, Division of Social Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and ¶Department of Medicine III, Osaka University Medical School, Osaka 565, Japan
Search for other works by this author on:
Kenji Igarashi,
Kenji Igarashi
From the *Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 590-02, Japan; ‡Shionogi Institute for Medical Science, Osaka 566, Japan; §Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan; ‖Department of Molecular Preventive Medicine, Division of Social Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and ¶Department of Medicine III, Osaka University Medical School, Osaka 565, Japan
Search for other works by this author on:
Hisatoshi Shida,
Hisatoshi Shida
From the *Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 590-02, Japan; ‡Shionogi Institute for Medical Science, Osaka 566, Japan; §Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan; ‖Department of Molecular Preventive Medicine, Division of Social Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and ¶Department of Medicine III, Osaka University Medical School, Osaka 565, Japan
Search for other works by this author on:
Hisashi Iizasa,
Hisashi Iizasa
From the *Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 590-02, Japan; ‡Shionogi Institute for Medical Science, Osaka 566, Japan; §Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan; ‖Department of Molecular Preventive Medicine, Division of Social Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and ¶Department of Medicine III, Osaka University Medical School, Osaka 565, Japan
Search for other works by this author on:
Nobuaki Yoshida,
Nobuaki Yoshida
From the *Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 590-02, Japan; ‡Shionogi Institute for Medical Science, Osaka 566, Japan; §Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan; ‖Department of Molecular Preventive Medicine, Division of Social Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and ¶Department of Medicine III, Osaka University Medical School, Osaka 565, Japan
Search for other works by this author on:
Osamu Yoshie,
Osamu Yoshie
From the *Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 590-02, Japan; ‡Shionogi Institute for Medical Science, Osaka 566, Japan; §Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan; ‖Department of Molecular Preventive Medicine, Division of Social Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and ¶Department of Medicine III, Osaka University Medical School, Osaka 565, Japan
Search for other works by this author on:
Tadamitsu Kishimoto,
Tadamitsu Kishimoto
From the *Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 590-02, Japan; ‡Shionogi Institute for Medical Science, Osaka 566, Japan; §Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan; ‖Department of Molecular Preventive Medicine, Division of Social Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and ¶Department of Medicine III, Osaka University Medical School, Osaka 565, Japan
Search for other works by this author on:
Takashi Nagasawa
Takashi Nagasawa
From the *Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 590-02, Japan; ‡Shionogi Institute for Medical Science, Osaka 566, Japan; §Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan; ‖Department of Molecular Preventive Medicine, Division of Social Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and ¶Department of Medicine III, Osaka University Medical School, Osaka 565, Japan
Search for other works by this author on:
Kazunobu Tachibana
,
Toshihiro Nakajima
,
Akihiko Sato
,
Kenji Igarashi
,
Hisatoshi Shida
,
Hisashi Iizasa
,
Nobuaki Yoshida
,
Osamu Yoshie
,
Tadamitsu Kishimoto
,
Takashi Nagasawa
From the *Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 590-02, Japan; ‡Shionogi Institute for Medical Science, Osaka 566, Japan; §Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan; ‖Department of Molecular Preventive Medicine, Division of Social Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan; and ¶Department of Medicine III, Osaka University Medical School, Osaka 565, Japan
Address correspondence to Takashi Nagasawa, Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 590-02, Japan.
Drs. Tachibana and Nakajima contributed equally to this work.
Received:
January 30 1997
Revision Received:
March 06 1997
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1997
J Exp Med (1997) 185 (10): 1865–1870.
Article history
Received:
January 30 1997
Revision Received:
March 06 1997
Citation
Kazunobu Tachibana, Toshihiro Nakajima, Akihiko Sato, Kenji Igarashi, Hisatoshi Shida, Hisashi Iizasa, Nobuaki Yoshida, Osamu Yoshie, Tadamitsu Kishimoto, Takashi Nagasawa; CXCR4/fusin Is Not a Species-specific Barrier in Murine Cells for HIV-1 Entry. J Exp Med 19 May 1997; 185 (10): 1865–1870. doi: https://doi.org/10.1084/jem.185.10.1865
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
