A subtractive analysis of peptides eluted from major histocompatibility complex (MHC) class I human histocompatibility leukocyte antigen (HLA)-A2.1 molecules purified from either human immunodeficiency virus type-1 (HIV-1)-infected or uninfected cells was performed using micro high-performance liquid chromatography and mass spectrometry. Three peptides unique to infected cells were identified and found to derive from a single protein, human vinculin, a structural protein not known to be involved in viral pathogenesis. Molecular and cytofluorometric analyses revealed vinculin mRNA and vinculin protein overexpression in B and T lymphocytes from HIV-1-infected individuals. Vinculin peptide-specific CTL activity was readily elicited from peripheral blood lymphocytes of the majority of HLA-A2.1+, HIV+ patients tested. Our observations suggest that atypical vinculin expression and MHC class I-mediated presentation of vinculin-derived peptides accompany HIV infection of lymphoid cells in vivo, with a resultant induction of antivinculin CTL in a significant portion of HIV+ (HLA-A2.1+) individuals.
Autoreactive cytotoxic T lymphocytes in human immunodeficiency virus type 1-infected subjects.
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F di Marzo Veronese, D Arnott, V Barnaba, D J Loftus, K Sakaguchi, C B Thompson, S Salemi, C Mastroianni, A Sette, J Shabanowitz, D F Hunt, E Appella; Autoreactive cytotoxic T lymphocytes in human immunodeficiency virus type 1-infected subjects.. J Exp Med 1 June 1996; 183 (6): 2509–2516. doi: https://doi.org/10.1084/jem.183.6.2509
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