Cytotoxic T cells (CTL) recognize short peptide epitopes presented by class I glycoproteins encoded by the major histocompatibility complex (MHC). It is not yet known whether peptides containing posttranslationally modified amino acids can also be recognized by CTL. To address this issue, we have studied the immunogenicity and recognition of a glycopeptide carrying an O-linked N-acetylglucosamine (GlcNAc) monosaccharide-substituted serine residue. This posttranslational modification is catalyzed by a recently described cytosolic glycosyltransferase. We show that glycosylation does not affect peptide binding to MHC class I and that glycopeptides can elicit a strong CTL response that is glycopeptide specific. Furthermore, glycopeptide recognition by cytotoxic T cells is dependent on the chemical structure of the glycan as well as its position within the peptide.
Article|
August 01 1994
Recognition of carbohydrate by major histocompatibility complex class I-restricted, glycopeptide-specific cytotoxic T lymphocytes.
J S Haurum,
J S Haurum
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
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G Arsequell,
G Arsequell
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
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A C Lellouch,
A C Lellouch
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
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S Y Wong,
S Y Wong
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
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R A Dwek,
R A Dwek
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
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A J McMichael,
A J McMichael
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
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T Elliott
T Elliott
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
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J S Haurum
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
G Arsequell
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
A C Lellouch
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
S Y Wong
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
R A Dwek
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
A J McMichael
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
T Elliott
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1994) 180 (2): 739–744.
Citation
J S Haurum, G Arsequell, A C Lellouch, S Y Wong, R A Dwek, A J McMichael, T Elliott; Recognition of carbohydrate by major histocompatibility complex class I-restricted, glycopeptide-specific cytotoxic T lymphocytes.. J Exp Med 1 August 1994; 180 (2): 739–744. doi: https://doi.org/10.1084/jem.180.2.739
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