We describe mice that express a transgenic T cell receptor alpha/beta (TCR-alpha/beta) specific for peptide 111-119 from influenza hemagglutinin presented by I-Ed class II major histocompatibility complex (MHC) molecules. The transgenic TCR is expressed on CD4+8- as well as CD4-8+ mature T cells even in mice that are deficient in rearrangement or do not express endogenous TCR-alpha genes. The CD4-8+ T cells require I-Ed class II MHC molecules for positive selection and can be activated to proliferate and to kill by I-Ed molecules presenting the relevant peptide. Full maturation of these cells, however, also requires the presence of class I MHC molecules. The results are compatible with the notion that T cell maturation requires multiple receptor-ligand interactions and establish an exception to the rule that class II-restricted TCRs are exclusively expressed by mature CD4+8- cells.
Thymic selection of CD8+ single positive cells with a class II major histocompatibility complex-restricted receptor.
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J Kirberg, A Baron, S Jakob, A Rolink, K Karjalainen, H von Boehmer; Thymic selection of CD8+ single positive cells with a class II major histocompatibility complex-restricted receptor.. J Exp Med 1 July 1994; 180 (1): 25–34. doi: https://doi.org/10.1084/jem.180.1.25
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