Ly-49 is a recently identified cell surface molecule expressed on a subpopulation of natural killer (NK) cells and certain T lymphomas. It has been suggested, based on gene transfection and antibody blocking studies, that Ly-49 is a negative regulator of NK lytic activity, possibly through an interaction with target cell class I molecules. However, it has not been demonstrated that class I molecules indeed serve as ligands for Ly-49. We have found that T lymphomas expressing Ly-49 bind isolated class I major histocompatibility complex (MHC) molecules but not class II molecules immobilized on plastic. Adhesion to class I molecules was not found with T lymphomas lacking Ly-49 expression. The Ly-49 expressing EL4 lymphoma bound Dd, Dk, and Kb, but not Kd, Kk, or Db, thus demonstrating a restricted pattern of class I adhesion. The observed cell adhesion was class I density dependent, and binding to Dd and Dk was extensively inhibited by the A1 monoclonal antibody directed against Ly-49. These results provide direct evidence for Ly-49 serving as a receptor for a subset of class I MHC molecules.
Article| March 01 1994
Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules.
K P Kane
Department of Immunology, University of Alberta, Edmonton, Canada.
J Exp Med (1994) 179 (3): 1011–1015.
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K P Kane; Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules.. J Exp Med 1 March 1994; 179 (3): 1011–1015. doi: https://doi.org/10.1084/jem.179.3.1011
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