Very late antigens VLA-1, VLA-2, VLA-3, and VLA-6, belonging to the beta 1 subfamily of integrins, have been identified as receptors for different binding domains of laminin (LM). We have detected VLA-6, but not VLA-1 and VLA-2 on a subset (50-70%) of fresh peripheral blood CD3-, CD16+, CD56+ human natural killer (NK) cells by immunofluorimetric and biochemical analysis. Binding assays performed on LM-coated plates showed that 10-15% of NK cells spontaneously adhere to LM, and this adhesion is mediated by VLA-6. Activation of NK cells through CD16 triggering or by phorbol ester results in a rapid increase of adhesion to LM, which is still mediated by VLA-6. The enhanced adhesiveness is not associated with changes in beta 1 LM receptor expression, while it correlates with changes in the phosphorylation status of alpha 6 subunit. The expression of VLA-6 on NK cells and the modulation of its avidity by activating stimuli may be relevant for NK cell migration and tissue location during inflammation or immune response.
Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6.
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A Gismondi, F Mainiero, S Morrone, G Palmieri, M Piccoli, L Frati, A Santoni; Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6.. J Exp Med 1 November 1992; 176 (5): 1251–1257. doi: https://doi.org/10.1084/jem.176.5.1251
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