An inhibitor-proteinase complex consisting of human alpha 1-PI and human leukocyte elastase is chemotactic for human neutrophils. The chemotactic activity is optimal at 1 nM and is associated only with the alpha 1-PI portion of the complex. Neither HLE in the complex, free HLE, nor native alpha 1-PI possesses chemotactic activity for human neutrophils. alpha 1-PI in complex is hydrolyzed at the Met-358-Ser-359 bond. The chemotactic activity is associated with the Mr 4,200 fragment of alpha 1-PI that has Ser-359 as its NH2 terminus. The region of the HLE-alpha 1-PI complex that stimulates chemotaxis appears to be the same as that of the Mr 4,200 fragment generated by hydrolysis of the Pro-357-Met-358 bond during proteolytic inactivation of alpha 1-PI. The data suggest the presence of a neutrophil surface receptor bound by alpha 1-PI after the formation of a complex with HLE or after proteolytic degradation. This receptor may play a role in clearance of these modified alpha 1-PI molecules.
Article|
May 01 1988
The inhibitory complex of human alpha 1-proteinase inhibitor and human leukocyte elastase is a neutrophil chemoattractant.
M J Banda,
M J Banda
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
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A G Rice,
A G Rice
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
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G L Griffin,
G L Griffin
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
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R M Senior
R M Senior
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
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M J Banda
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
A G Rice
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
G L Griffin
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
R M Senior
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1988) 167 (5): 1608–1615.
Citation
M J Banda, A G Rice, G L Griffin, R M Senior; The inhibitory complex of human alpha 1-proteinase inhibitor and human leukocyte elastase is a neutrophil chemoattractant.. J Exp Med 1 May 1988; 167 (5): 1608–1615. doi: https://doi.org/10.1084/jem.167.5.1608
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