We found that the synthesis of IgE anti-Ars antibodies is strongly inhibited by the presence of secondary non-IgE-producing cells that are specific for the Ars hapten. Such B cells can be induced by inoculation of a protein-Ars conjugate in CFA. The effect is seen after inoculation of OVA-Ars in CFA followed by KLH-Ars in alum, or, more convincingly, after adoptive transfer of B cells induced by antigen in CFA. Dose-response data indicated that inhibition can be effected by B cells containing noninhibitory numbers of contaminating T cells. Possible synergistic effects of carrier-specific regulatory T cells were ruled out by using a different protein carrier for immunization of donor and recipient mice. The effect was shown to be specific for the hapten used for immunization of donor mice.
Article| December 01 1986
Inhibition of an IgE response by secondary B cells of a different isotype.
M F Gurish
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1986) 164 (6): 2018–2030.
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S Haba, M F Gurish, A Nisonoff; Inhibition of an IgE response by secondary B cells of a different isotype.. J Exp Med 1 December 1986; 164 (6): 2018–2030. doi: https://doi.org/10.1084/jem.164.6.2018
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