Factors known to be important in producing protein-reactive peptide antibodies include the accessibility of the region from which the peptide sequence is derived, the hydrophilic-phobic character of the sequence, and the length of the peptide. The data presented here indicate that the orientation of the peptide coupled to a carrier protein also influences the binding pattern of peptide antibodies. An octapeptide, representing a sequence from the alpha chain of the human acetylcholine receptor, was coupled either through an N- or C-terminal cysteine-glycine-glycine linker to a carrier protein and used to immunize rabbits. The resulting antisera reacted at comparable titers to the uncoupled immunizing peptides, but did not crossreact with the identical but opposite-linked peptide. Characterization of the binding to other homologous peptides showed that immunization with the N-terminal-linked peptide induced antibodies reactive specifically with the C-terminal amino acid(s). Immunization with the C-linked peptide resulted in antibodies reactive with a site of the peptide near the C-terminus.
Article| October 01 1986
Peptides as antigens. Importance of orientation.
M B Oldstone
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1986) 164 (4): 1344–1349.
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T Dyrberg, M B Oldstone; Peptides as antigens. Importance of orientation.. J Exp Med 1 October 1986; 164 (4): 1344–1349. doi: https://doi.org/10.1084/jem.164.4.1344
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